Recombinant Drosophila melanogaster 26S proteasome non-ATPase regulatory subunit 4 (Pros54)

Code CSB-YP018908DLU
MSDS
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Source Yeast
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Code CSB-EP018908DLU
MSDS
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Source E.coli
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Code CSB-EP018908DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018908DLU
MSDS
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Source Baculovirus
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Code CSB-MP018908DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rpn10
Uniprot No.
Alternative Names
Rpn10; PROS-54; Pros54; CG7619; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; 54 kDa subunit of mu particle; Multiubiquitin chain-binding protein; p54
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-396
Target Protein Sequence
MVLESTMICF DNSDFQRNGD YFPTRLIVQR DGINLVCLTK LRSNPENNVG LMTLSNTVEV LATLTSDAGR IFSKMHLVQP KGEINLLTGI RIAHLVLKHR QGKNHKMRIV VFVGSPINHE EGDLVKQAKR LKKEKVNVDI VSFGDHGNNN EILTAFINAL NGKDGTGSHL VSVPRGSVLS DALLSSPIIQ GEDGMGGAGL GGNVFEFGVD PNEDPELALA LRVSMEEQRQ RQESEQRRAN PDGAPPTGGD AGGGGGVSGS GPGNEESAGA ENEANTEEAM LQRALALSTE TPEDNLPDFA NMTEEEQIAF AMQMSMQDAP DDSVTQQAKR PKTDEANAPM DVDEDYSEVI GDPAFLQSVL ENLPGVDPQS EAVRDAVGSL NKDKDKKSDG KDSQKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds and presumably selects ubiquitin-conjugates for destruction.
Gene References into Functions
  1. S5a/Rpn10/p54 deletion caused larval-pupal polyphasic lethality, multiple mitotic defects, the accumulation of higher multimers of ubiquitinated proteins and a huge accumulation of defective 26S proteasome particles. PMID: 12584246
Protein Families
Proteasome subunit S5A family
Database Links

KEGG: dme:Dmel_CG7619

STRING: 7227.FBpp0078024

UniGene: Dm.23542

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