Recombinant Drosophila melanogaster Achaete-scute complex protein T4 (sc)

Code CSB-YP318009DLU
MSDS
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Source Yeast
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Code CSB-EP318009DLU
MSDS
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Source E.coli
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Code CSB-EP318009DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318009DLU
MSDS
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Source Baculovirus
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Code CSB-MP318009DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sc
Uniprot No.
Alternative Names
sc; sis-b; T4; CG3827Achaete-scute complex protein T4; Protein scute
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-345
Target Protein Sequence
MKNNNNTTKS TTMSSSVLST NETFPTTINS ATKIFRYQHI MPAPSPLIPG GNQNQPAGTM PIKTRKYTPR GMALTRCSES VSSLSPGSSP APYNVDQSQS VQRRNARERN RVKQVNNSFA RLRQHIPQSI ITDLTKGGGR GPHKKISKVD TLRIAVEYIR RLQDLVDDLN GGSNIGANNA VTQLQLCLDE SSSHSSSSST CSSSGHNTYY QNTISVSPLQ QQQQLQRQQF NHQPLTALSL NTNLVGTSVP GGDAGCVSTS KNQQTCHSPT SSFNSSMSFD SGTYEGVPQQ ISTHLDRLDH LDNELHTHSQ LQLKFEPYEH FQLDEEDCTP DDEEILDYIS LWQEQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. Also involved in sex determination and dosage compensation.
Gene References into Functions
  1. Results show that both Esg and Sc bind to a common promoter region of pros. Moreover, antagonistic activity between Esg and Sc controls the expression status of Pros in stem cells, thereby, specifying whether stem cells remain undifferentiated or commit to enteroendocrine cell differentiation. PMID: 28428611
  2. Data show that bHLHb22 protein daughterless (Da) binding alters the turnover parameters of bHLHc28 protein scute (Sc). PMID: 25694512
  3. interchanging E(Ato) and E(Sc) sites within enhancers almost abolishes enhancer activity PMID: 15485919
  4. the Sc C-terminal domain is required for E(spl) recruitment in an enhancer context-dependent fashion, suggesting that in some enhancers alternative interaction surfaces can be used to recruit E(spl) proteins PMID: 15507447
Tissue Specificity
L(1)SC, SC and AC strongly label the presumptive stomatogastric nervous system, while ASE is more prominent in the presumptive procephalic lobe. Associates with the somatic nuclei through nuclear cycles 9 and 10. During nuclear cycle 11 distributes unifor
Database Links

KEGG: dme:Dmel_CG3827

STRING: 7227.FBpp0070072

UniGene: Dm.26417

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