Recombinant Drosophila melanogaster Anamorsin homolog (l (2)35Bg)

Code CSB-YP895563DLU
MSDS
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Source Yeast
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Code CSB-EP895563DLU
MSDS
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Source E.coli
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Code CSB-EP895563DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895563DLU
MSDS
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Source Baculovirus
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Code CSB-MP895563DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CIAPIN1
Uniprot No.
Alternative Names
CIAPIN1; l(2)35Bg; CG4180Anamorsin homolog; Fe-S cluster assembly protein DRE2 homolog
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-248
Target Protein Sequence
MENFKGLQKS LYIWTDSADL DKRVEQLKAA TGGDVALENV HRLSFSSYAN SSFDLIVIEC AQLTDSYVKL LHMLKPSGKL HLVSYIGPAA SLLQEIKLSG FINCREDSPD ALTAEKPGYE TGSSARLSFA KKNASAVNVW KISGDDEELI DEEELLDEED KQKPDPAGLR VCSTTGKRKA CKNCSCGLAE ELETEKQSQK ATENAKSSCG NCYLGDAFRC STCPYLGMPA FKPGEKVQLA DNLLKSDI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.
Gene References into Functions
  1. D-CIAPIN1 is essential for egg chamber development and is required for follicle cell proliferation and survival PMID: 23444317
Subcellular Location
Cytoplasm. Mitochondrion intermembrane space.
Protein Families
Anamorsin family
Database Links

KEGG: dme:Dmel_CG4180

STRING: 7227.FBpp0080284

UniGene: Dm.1622

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