Recombinant Drosophila melanogaster Aurora kinase B (ial)

Code CSB-YP002458DLU
MSDS
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Source Yeast
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Code CSB-EP002458DLU
MSDS
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Source E.coli
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Code CSB-EP002458DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002458DLU
MSDS
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Source Baculovirus
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Code CSB-MP002458DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ial
Uniprot No.
Alternative Names
ial; CG6620; Aurora kinase B; EC 2.7.11.1; IPL1/Aurora-like protein kinase; Serine/threonine-protein kinase Ial; Serine/threonine-protein kinase aurora-B
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-329
Target Protein Sequence
MTLSRAKHAN RNHLPHLLAK VPEEHQEPIK NMCLKMMSHD AYGQPYDWSP RDFEMGAHLG RGKFGRVYLA RERHSHYLVA MKVMFKEELR KGCVQRQVLR EIEIQSRLKH PHILRLLTWF HDESRIYLAL EIASEGELFK HLRGAPNHRF DEPRSAKYTY QVANALNYCH LNNVIHRDLK PENILLTSTD DLKLADFGWS AHTPNNKRRT LCGTLDYLPP EMVDGNSYDD SVDQWCLGIL CYEFVVGCPP FESNSTESTY SKIRRMEISY PSHLSKGCKE LIGGLLRKES KGRITLVDVM THYWVKAGMA ERELQLQKRE RGKENTARN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine/threonine-protein kinase which mediates both meiotic and mitotic chromosome segregation. Required for histone H3 'Ser-10' phosphorylation. Phosphorylates mei-S332 within residues 124-126 and stabilizes its association with centromeres during meiosis.
Gene References into Functions
  1. Regulation of Polo by Aurora B and Map205 is required for cytokinesis. PMID: 25332165
  2. We propose that mutual inhibitions between Aurora B and Cyclin B regulate the duration of abscission and thereby the number of sister cells in each cyst. PMID: 23948252
  3. Aurora B kinase activity is not required during contractile ring ingression, providing insight into the mechanism of cytokinesis. PMID: 20804174
  4. Aurora B interacts with and requires the Cdc37/Hsp90 complex for its stability. PMID: 12374737
  5. This protein accumulates at the mid-zones of chromosome-free spindles. PMID: 12654903
  6. INCENP binds to the cohesion protector protein MEI-S332, which is also an excellent in vitro substrate for Aurora B kinase. PMID: 16824953

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Subcellular Location
Chromosome. Cytoplasm, cytoskeleton. Midbody. Note=Meiotic and mitotic chromosomes. During each division, relocates to the midbody microtubules.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
Database Links

KEGG: dme:Dmel_CG6620

STRING: 7227.FBpp0079769

UniGene: Dm.3103

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