Recombinant Drosophila melanogaster Borealin (borr)

Code CSB-YP896113DLU
MSDS
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Source Yeast
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Code CSB-EP896113DLU
MSDS
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Source E.coli
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Code CSB-EP896113DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896113DLU
MSDS
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Source Baculovirus
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Code CSB-MP896113DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
borr
Uniprot No.
Alternative Names
borr; CG4454Borealin; Aurora borealis protein; Borealin-related
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-315
Target Protein Sequence
MPRTKIPKNS KRNREVANRE EKVRLYELKM DSRLISIDSL ETKFLSAVDH QVKTLLGQVQ KELANLKMPE VLRLFEELDR FSDFKASDQT QLLASMSMSG SAIEGHAPSA TGSRNDEEDS SIGASGGSIL AAHTGSLLRS TKAMRTPGPL HSARARRARR SRSACGDLNI LHSAKPPSIS SSSSSSRNSR SKLRTPMASR AKAFSADRTP LKQKQMRSGS PTTPPMAFLR YPKPGEVALS KYGSPMVAQV MPDKFANVNI PIRNGVLSLR PKKLDADEVE SNLLENLDED TLNQIKTLHE NLQMIVNKAS QAVFK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of embryonic mitosis. The CPC complex has essential functions at the centromere for ensuring sister chromatid cohesion, recruitment of the CPC to kinetochores, and chromosome alignment and segregation. There is no function in meiotic histone phosphorylation or spindle formation.
Gene References into Functions
  1. Borr interacts and colocalizes with the ESCRT-III component Shrb in Drosophila cells. PMID: 22724069
Subcellular Location
Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with and is required for the correct localization of the CPC components Aurora B kinase and Incenp in mitotic cells. Is absent from spermatocytes undergoing meiosis.
Protein Families
Borealin family
Tissue Specificity
Ubiquitously expressed in the early embryo. Expression is restricted to the ventral nerve cord and brain during later embryonic stages.
Database Links

KEGG: dme:Dmel_CG4454

STRING: 7227.FBpp0079417

UniGene: Dm.21364

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