Recombinant Drosophila melanogaster COP9 signalosome complex subunit 2 (alien)

Code CSB-YP846397DLU
MSDS
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Source Yeast
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Code CSB-EP846397DLU
MSDS
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Source E.coli
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Code CSB-EP846397DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846397DLU
MSDS
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Source Baculovirus
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Code CSB-MP846397DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
alien
Uniprot No.
Alternative Names
alien; CSN2; CG9556COP9 signalosome complex subunit 2; Dch2; Signalosome subunit 2; Alien protein
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-444
Target Protein Sequence
MSDNDDDFMC DDDEDYGLEY SEDSNSEPDV DLENQYYNSK ALKEEEPKAA LASFQKVLDL ENGEKGEWGF KALKQMIKIN FRLCNYDEMM VRYKQLLTYI KSAVTRNHSE KSINSILDYI STSKNMALLQ NFYETTLDAL RDAKNDRLWF KTNTKLGKLY FDRSDFTKLQ KILKQLHQSC QTDDGEDDLK KGTQLLEIYA LEIQMYTVQK NNKKLKALYE QSLHIKSAIP HPLIMGVIRE CGGKMHLREG EFEKAHTDFF EAFKNYDESG SPRRTTCLKY LVLANMLMKS GINPFDSQEA KPYKNDPEIL AMTNLVNSYQ NNDINEFETI LRQHRSNIMA DQFIREHIED LLRNIRTQVL IKLIRPYKNI AIPFIANALN IEPAEVESLL VSCILDDTIK GRIDQVNQVL QLDKINSSAS RYNALEKWSN QIQSLQFAVV QKMA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. The CSN complex plays an essential role in oogenesis and embryogenesis and is required for proper photoreceptor R cell differentiation and promote lamina glial cell migration or axon targeting. It also promotes Ubl-dependent degradation of cyclin E (CycE) during early oogenesis.
Gene References into Functions
  1. Alien seems to modulate nucleosome assembly activity. This suggests that Alien is acting on chromatin not only through recruitment of histone-modifying activities, but also through enhancing nucleosome assembly. PMID: 18174916
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
CSN2 family
Tissue Specificity
Expressed during embryonic stages 11-14 in the muscle attachment sites (apodemes); pharynx attachment to the roof of the mouth and in the epidermis of the head for the dorsal and ventral prothoracic pharyngeal muscle attachment. From stage 16 onwards expr
Database Links

KEGG: dme:Dmel_CG9556

STRING: 7227.FBpp0303869

UniGene: Dm.20642

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