Recombinant Drosophila melanogaster Casein kinase II subunit alpha (CkIIalpha)

Code CSB-YP006072DLU
MSDS
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Source Yeast
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Code CSB-EP006072DLU
MSDS
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Source E.coli
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Code CSB-EP006072DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006072DLU
MSDS
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Source Baculovirus
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Code CSB-MP006072DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CkIIalpha
Uniprot No.
Alternative Names
CkIIalpha; Cask-II-a; CG17520Casein kinase II subunit alpha; CK II subunit alpha; EC 2.7.11.1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
2-336
Target Protein Sequence
TLPSAARVY TDVNAHKPDE YWDYENYVVD WGNQDDYQLV RKLGRGKYSE VFEAINITTT EKCVVKILKP VKKKKIKREI KILENLRGGT NIITLLAVVK DPVSRTPALI FEHVNNTDFK QLYQTLTDYE IRYYLFELLK ALDYCHSMGI MHRDVKPHNV MIDHENRKLR LIDWGLAEFY HPGQEYNVRV ASRYFKGPEL LVDYQMYDYS LDMWSLGCML ASMIFRKEPF FHGHDNYDQL VRIAKVLGTE ELYAYLDKYN IDLDPRFHDI LQRHSRKRWE RFVHSDNQHL VSPEALDFLD KLLRYDHVDR LTAREAMAHP YFLPIVNGQM NPNNQQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May participate in Wnt signaling.
Gene References into Functions
  1. CK2alpha might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2alpha is a novel regulator of the active zone protein, BRP, in Drosophila. PMID: 24080510
  2. overexpression of Tik elicits ectopic bristles in N(+) flies and suppresses the retinal defects of the gain-of-function allele N(spl). PMID: 19536808
  3. Sequencing of full-length cDNA encoding subunits in platelets and megakaryocytic cells PMID: 12102635
  4. CK2 alpha binds and phosphorylates ribosomal protein L22 PMID: 12379220
  5. CK2alpha mutant flies show lengthened circadian period, decreased CK2 activity, and delayed nuclear entry of Per; these effects are probably direct, as CK2alpha specifically phosphorylates Per in vitro PMID: 12447397
  6. casein kinase II has a role in regulating cAMP-response element-binding protein DNA binding by phosphorylation at specific CK sites PMID: 14676200
  7. Dominant-negative CK2alpha induces potent effects on circadian rhythmicity. PMID: 18208335
  8. Results describe the isolation of temperature-sensitive alleles of Drosophila CK2alpha. PMID: 19039653

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Subcellular Location
Nucleus, nucleolus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
Database Links

KEGG: dme:Dmel_CG17520

STRING: 7227.FBpp0110433

UniGene: Dm.1750

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