Recombinant Drosophila melanogaster Chitinase-like protein Idgf4 (Idgf4)

Code CSB-YP892933DLU
MSDS
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Source Yeast
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Code CSB-EP892933DLU
MSDS
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Source E.coli
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Code CSB-EP892933DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892933DLU
MSDS
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Source Baculovirus
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Code CSB-MP892933DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Idgf4
Uniprot No.
Alternative Names
Idgf4; CG1780; Chitinase-like protein Idgf4; Imaginal disk growth factor protein 4
Species
Drosophila melanogaster (Fruit fly)
Expression Region
22-442
Target Protein Sequence
AGSHHLLCY YDGNSFVREG LSKLILTDLE PALQYCTHLV YGYAGINPSS NKLVSNNEKL DLDLGSSLFR QVTGLKRKYP ALKVLLSVGG DKDTVDPENN KYLTLLESSN ARIPFINSAH SLVKTYGFDG LDLGWQFPKN KPKKVHGSIG KFWKGFKKIF SGDHVVDEKA EEHKEAFTAL VRELKNAFRP DGYILGLSVL PNVNSSLFFD VPAIINNLDY VNLHTYDFQT PERNNEVADF PAPIYELNER NPEFNVNYQV KYWTGNRAPA AKINVGIATY GRAWKLTKDS GLTGLPPVAE ADGVAPAGTQ TQIPGLLSWP EVCAKLPNPA NQHLKGADGP LRKVGDPTKR FGSYAYRSAD DSGENGVWVG YEDPDTAAIK AEYVKREGLG GIAVVDLSFD DFRGGCTGHD KFPILRQVKS KL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cooperates with insulin-like peptides to stimulate the proliferation, polarization and motility of imaginal disk cells. May act by stabilizing the binding of insulin-like peptides to its receptor through a simultaneous interaction with both molecules to form a multiprotein signaling complex.
Gene References into Functions
  1. ifferential mass spectrometry analysis of these cells detected elevated levels of four of the six Idgf family members (Idgf1, Idgf2, Idgf4, and Idgf6) in flies mutant for bullwinkle (bwk), which encodes a transcription factor and is a known regulator of DA-tube morphogenesis. We show that, during oogenesis, dysregulation of Idgfs (either gain or loss of function) disrupts the formation of the DA tubes PMID: 28404605
Subcellular Location
Secreted. Note=Secreted in hemolymph. It is probably transported to target tissues via hemolymph.
Protein Families
Glycosyl hydrolase 18 family, IDGF subfamily
Tissue Specificity
Primarily expressed in yolk cells and fat body. In larvae, it is expressed in the imaginal ring, the salivary duct, large salivary gland cells and weakly expressed in imaginal disks. More strongly expressed than Idgf1 and Idgf3.
Database Links

KEGG: dme:Dmel_CG1780

STRING: 7227.FBpp0071328

UniGene: Dm.7207

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