Recombinant Drosophila melanogaster Chromatin-remodeling complex ATPase chain Iswi (Iswi), partial

Code CSB-YP634508DLU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP634508DLU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP634508DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP634508DLU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP634508DLU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Iswi
Uniprot No.
Alternative Names
Iswi; CG8625; Chromatin-remodeling complex ATPase chain Iswi; EC 3.6.4.-; CHRAC 140 kDa subunit; Nucleosome-remodeling factor 140 kDa subunit; NURF-140; Protein imitation swi
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Energy-transducing component of the chromatin-remodeling complexes NURF (nucleosome-remodeling factor), ACF (ATP-utilizing chromatin assembly and remodeling factor), and CHRAC (chromatin accessibility complex). NURF catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. It is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis.
Gene References into Functions
  1. the role of ISWI in the hnRNPshsromega interaction PMID: 28209301
  2. ISWI enables bi-directional transitions between two fundamentally different forms of chromatin. PMID: 26549447
  3. Insertion of flexible linkers at strategic sites in ISWI that disrupt a power stroke does not affect nucleosome sliding. PMID: 24113208
  4. In a purified system ISWI- and CHD1-type nucleosome remodelers have a clamping activity such that they not only generate regularly spaced nucleosome arrays but also generate constant spacing regardless of nucleosome density. PMID: 25733687
  5. The effect of H4 tail at lysine 16 acetylation on ISWI remodelling depends on the precise nature of the substrate. PMID: 24516652
  6. finding show all fundamental aspects of nucleosome-remodeling catalysis are contained within the compact ATPase domain of ISWI PMID: 23202585
  7. define two new, conserved and separate regulatory regions on Drosophila ISWI, termed AutoN and NegC, which negatively regulate ATP hydrolysis (AutoN) or the coupling of ATP hydrolysis to productive DNA translocation (NegC). PMID: 23143334
  8. This study revealed a novel conformation of an ISWI ATPase domain PMID: 22167269
  9. Loss of ISWI causes global defects in omega speckle organization. PMID: 21637796
  10. ISWI binds genes near their promoters causing specific alterations in nucleosome positioning at the level of the Transcription Start Site, and thus a role in higher eukaryote transcriptional regulation. PMID: 21448136
  11. ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic and biochemical analyses. PMID: 20194965
  12. results demonstrate that distinctly from its role in spindle assembly, RanGTP maintains spindle microtubules in anaphase through the local activation of ISWI and that this is essential for proper chromosome segregation PMID: 20008562
  13. ISWI recognizes an 'epitope' consisting of the DNA-bound H4 tail PMID: 11809876
  14. Histone acetylation reduces the ability of ISWI to interact with its substrate, couteracting chromatin compaction. PMID: 11882543
  15. ISWI is a 3'-5'-strand-specific, ATP-dependent DNA translocase that may be capable of forcing DNA over the surface of nucleosomes. PMID: 12612068
  16. Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI PMID: 14536084
  17. the roles of dom and iswi in germ line stem cell and somatic stem cell regulation PMID: 16322456
  18. Site-specific acetylation of Iswi by Gcn5. PMID: 17760996
  19. The chromatin remodeler Iswi directly represses Wg transcriptional targets. PMID: 18786525
  20. The work suggests that ISWI is a physiological target of PARP and that poly-ADP-ribosylation can be a new, important post-translational modification regulating the activity of ATP-dependent nucleosome remodelers. PMID: 18922045
  21. Findings suggest that ISWI regulates higher-order chromatin structure by modulating the interaction of H1 with interphase chromosomes. PMID: 19380479

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
SNF2/RAD54 helicase family, ISWI subfamily
Database Links

KEGG: dme:Dmel_CG8625

STRING: 7227.FBpp0086954

UniGene: Dm.2581

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1