Recombinant Drosophila melanogaster Ecdysone-induced protein 75B, isoform B (Eip75B), partial

Code CSB-YP321053DLU
MSDS
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Source Yeast
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Code CSB-EP321053DLU
MSDS
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Source E.coli
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Code CSB-EP321053DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321053DLU
MSDS
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Source Baculovirus
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Code CSB-MP321053DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Eip75B
Uniprot No.
Alternative Names
Eip75B; NR1D3; CG8127; Ecdysone-induced protein 75B; isoform B; E75-C; Nuclear receptor subfamily 1 group D member 3; isoform B
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Implicated in the regulation of ecdysone-triggered gene hierarchies. Probably plays a key role in mediating the regulation of the larval molt by 20-OH-ecdysone.
Gene References into Functions
  1. These results suggest that E75C, like UNF, is required for developmental regrowth, but not initial outgrowth of mushroom body gamma axons. PMID: 26771490
  2. Covalent attachment of heme to the protein moiety in an insect E75 nitric oxide sensor. PMID: 22946928
  3. FTZ-F1 functions as a competence factor by loading juvenile hormone (JH) signaling components to the promoter, thus facilitating the direct regulation of E75A gene expression by JH. PMID: 21832074
  4. nitric oxide is produced in the Drosophila prothoracic gland, where it acts via the nuclear receptor ecdysone-induced protein 75 (E75), reversing its ability to interfere with its heterodimer partner, Drosophila hormone receptor 3 (DHR3). PMID: 21715559
  5. the ligand binding pocket of the Drosophila nuclear receptor E75 contains a heme prosthetic group and may also function as a diatomic gas sensor PMID: 16051145
  6. E75 is a new example of a thiolate hemoprotein that may be involved in hormone synthesis regulation. PMID: 16893174

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR1 subfamily
Database Links

KEGG: dme:Dmel_CG8127

STRING: 7227.FBpp0297726

UniGene: Dm.21411

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