Recombinant Drosophila melanogaster Ejaculatory bulb-specific protein 3 (PebIII)

Ejaculatory bulb-specific protein 3 (EbpIII) is a protein found in the ejaculatory bulb and seminal fluid of Drosophila melanogaster [1]. It is a member of the chemosensory protein (CSP) gene family and is involved in environmental stimulus reception and resistance to insecticides [2]. EbpIII is also associated with olfactory function, post-mating behavior, and mating plug formation [3][4]. In Drosophila, it is known that ejaculatory bulb proteins, including EbpIII, play a crucial role in postmating interactions and mating plug formation [5][6]. Furthermore, the expression of EbpIII is specific to the ejaculatory bulbs of adult males [7].

EbpIII is also linked to sex determination and differentiation in insects, as it has been identified as one of the genes related to sex determination and differentiation in the sugarcane borer [8]. Additionally, EbpIII is specifically expressed in the ejaculatory bulb and is associated with male reproductive function and postmating interactions [9][10][11].

References:
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[6] F. Avila, A. Cohen, F. Ameerudeen, D. Duneau, S. Suresh, A. Matteiet al., "Retention of ejaculate by drosophila melanogaster females requires the male-derived mating plug protein pebme", Genetics, vol. 200, no. 4, p. 1171-1179, 2015. https://doi.org/10.1534/genetics.115.176669
[7] G. Yenikolopov, B. Kuzin, N. Evgen'ev, M. Ludwig, L. Korochkin, & G. Georgiev, "The cloning and expression of the gene encoding organ-specific esterase s from the genome of drosophila virilis.", The Embo Journal, vol. 2, no. 1, p. 1-7, 1983. https://doi.org/10.1002/j.1460-2075.1983.tb01371.x
[8] A. Li, W. He, J. Wei, Z. Chen, F. Liao, C. Qinet al., "Transcriptome profiling reveals genes related to sex determination and differentiation in sugarcane borer (chilo sacchariphagus bojer)", Insects, vol. 13, no. 6, p. 500, 2022. https://doi.org/10.3390/insects13060500
[9] F. Avila, L. Sirot, B. LaFlamme, C. Rubinstein, & M. Wolfner, "Insect seminal fluid proteins: identification and function", Annual Review of Entomology, vol. 56, no. 1, p. 21-40, 2011. https://doi.org/10.1146/annurev-ento-120709-144823
[10] A. Cohen and M. Wolfner, "Dynamic changes in ejaculatory bulb size during drosophila melanogaster aging and mating", Journal of Insect Physiology, vol. 107, p. 152-156, 2018. https://doi.org/10.1016/j.jinsphys.2018.04.005
[11] T. Chapman, "Seminal fluid-mediated fitness traits in drosophila", Heredity, vol. 87, no. 5, p. 511-521, 2001. https://doi.org/10.1046/j.1365-2540.2001.00961.x