Recombinant Drosophila melanogaster Exportin-1 (emb), partial

Code CSB-YP874697DLU
MSDS
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Source Yeast
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Code CSB-EP874697DLU
MSDS
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Source E.coli
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Code CSB-EP874697DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874697DLU
MSDS
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Source Baculovirus
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Code CSB-MP874697DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
emb
Uniprot No.
Alternative Names
emb; Crm1; CG13387; Exportin-1; Chromosome region maintenance 1 protein; Protein embargoed
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for the leucine-rich nuclear export signal (NES). Binds cooperatively to the NES on its target protein and to the small GTPase Ran in its active GTP-bound form. Involved in the export of dl, RpS2 and the pre-40S ribosome from the nucleus to the cytoplasm. Plays an important role in nuclear pore assembly by mediating nucleoporin condensation and biogenesis of annulate lamellae. Required for the function or maintenance of certain tissues such as brain and gut.
Gene References into Functions
  1. two newly identified factors that restrict diverse viruses, dXPO1 and dRUVBL1 contributed to antiviral defense at the organismal level in adult flies PMID: 24550726
  2. HPO promotes the translocation of SD to the cytoplasm in a CRM1-dependent manner PMID: 23271049
  3. Exportin-1 is a nuclear export receptor for expanded polyQ containing proteins. PMID: 21300695
  4. Nuclear localization of the ecdysteroid receptor (EcR) is increased in HeLa cells if exportin-1 (CRM1) is knocked down by siRNA against exportin. PMID: 21125558
  5. Data show that downregulation of YAN involves CRM1-mediated nuclear export, and that MAE is involved in MAPK phosphorylationof YAN. PMID: 12538513
  6. A major function of DNup88 is to anchor DNup214 and CRM1 on the nuclear envelope and thereby attenuate NES-mediated nuclear export. PMID: 14638854
  7. Phosphorylation of Yan favors Crm1 in this competition and counteracts inhibition of nuclear export by Mae PMID: 16027171
  8. two functional nuclear export signals are in the Sima basic helix-loop-helix (bHLH) domain and promote CRM1-dependent nuclear export PMID: 18332128

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Subcellular Location
Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.
Protein Families
Exportin family
Tissue Specificity
High expression observed in the developing embryonic brain, hind gut and posterior spiracles shortly before dorsal closure; and in the ventral nerve cord, midgut and somatic musculature shortly after dorsal closure. Expression increases when the tissue is
Database Links

KEGG: dme:Dmel_CG13387

STRING: 7227.FBpp0079278

UniGene: Dm.3346

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