Recombinant Drosophila melanogaster FMRFamide-related peptides (Fmrf)

Code CSB-YP016032DLU
MSDS
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Source Yeast
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Code CSB-EP016032DLU
MSDS
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Source E.coli
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Code CSB-EP016032DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016032DLU
MSDS
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Source Baculovirus
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Code CSB-MP016032DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FMRFa
Uniprot No.
Alternative Names
FMRFa; Fmrf; CG2346FMRFamide-related peptides [Cleaved into: FMRFamide A; Corticotropin-releasing factor-like; AAMDRY-amide; DPKQDFMRF-amide; TPAEDFMRF-amide; SDNFMRF-amide; SPKQDFMRF-amide; PDNFMRF-amide; SAPQDFVRS-amide; MDSNFIRF-amide]
Species
Drosophila melanogaster (Fruit fly)
Expression Region
106-114
Target Protein Sequence
SVQDN FMHF
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
In insects, FMRFamide and related peptides have modulatory actions at skeletal neuromuscular junctions, and peptides that are immunologically related to FMRFamide are released into the circulation from neurohemal organs.
Gene References into Functions
  1. Animals mutant for either FMRFamide or for the FMRFamide receptor (FR) have a reduced recovery sleep in response to heat stress. PMID: 25668617
  2. DPKQDFMRFamide induces contractions via a G-protein coupled FMRFamide receptor in muscle cells but does not appear to act via cAMP, cGMP, CaMKII or arachidonic acid. PMID: 24526728
  3. Klu is expressed specifically in the postmitotic Ap4/FMRFa neuron, promoting its differentiation through the initiation of BMP signaling PMID: 23633512
  4. DPKQDFMRFamide modulates both synaptic function and behavior through coincident detection mediated by FMRF receptor and dromyosuppressin receptor-2. PMID: 21048131
  5. Here, we review FMRFamide-containing peptides encoded by the D. melanogaster FMRFamide gene in order to review the data on the expression, regulation, and activity of these peptides as well as acknowledge endeavors required to elucidate FaRP signaling. PMID: 11814637
  6. Supplying FMRFa in neurosecretory cells partially rescues the wit lethal phenotype. PMID: 14507784
  7. hybridization in situ shows a lack of co-localization of mRNAs for the circadian neuropeptide PDF and FMRFamide in brains of the flies Musca domestica and Drosophila melanogaster PMID: 17426155

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Subcellular Location
Secreted.
Protein Families
FARP (FMRFamide related peptide) family
Database Links

KEGG: dme:Dmel_CG2346

STRING: 7227.FBpp0087471

UniGene: Dm.7156

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