Recombinant Drosophila melanogaster GTPase-activating protein (Gap1), partial

Code CSB-YP344138DLU
MSDS
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Source Yeast
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Code CSB-EP344138DLU
MSDS
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Source E.coli
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Code CSB-EP344138DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344138DLU
MSDS
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Source Baculovirus
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Code CSB-MP344138DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RasGAP1
Uniprot No.
Alternative Names
RasGAP1; Gap1; CG6721GTPase-activating protein; Ras GTPase-activating protein 1
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibitory regulator of the Ras-cyclic AMP pathway. May function as a negative regulator of Ras85D/Ras1 in the sev signaling pathway. Acts cell autonomously in cone cell precursors as a negative regulator of R7 photoreceptor cell determination.
Gene References into Functions
  1. the PlexA GAP domain is phosphorylated by the cAMP-dependent protein kinase (PKA). This PlexA phosphorylation generates a specific binding site for 14-3-3epsilon, a phospho-binding protein that we find to be necessary for axon guidance PMID: 22500634
  2. Neurofibromatosis type 1 (NF1) is among the most common genetic disorders of humans and is caused by loss of neurofibromin, a large and highly conserved protein whose only known function is to serve as a GTPase-Activating Protein (GAP) for Ras. PMID: 17114577
Tissue Specificity
In third instar larvae eye imaginal disk, expressed in cells posterior to the morphogenetic furrow, in all photoreceptor and cone cell precursors as well as in still uncommitted cells.
Database Links

KEGG: dme:Dmel_CG6721

STRING: 7227.FBpp0303201

UniGene: Dm.919

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