Recombinant Drosophila melanogaster High mobility group protein DSP1 (Dsp1)

Code CSB-YP638766DLU
MSDS
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Source Yeast
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Code CSB-EP638766DLU
MSDS
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Source E.coli
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Code CSB-EP638766DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP638766DLU
MSDS
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Source Baculovirus
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Code CSB-MP638766DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dsp1
Uniprot No.
Alternative Names
Dsp1; ssrp2; CG12223; High mobility group protein DSP1; Protein dorsal switch 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-393
Target Protein Sequence
MEHFHQIQQT IQHYQQQLAA QQQQQVQQQQ LQQHQVVVQQ NQQQAHQNSS NTTAGVGTQQ LFTYKMASSF PNPATTMAQV VATSNAAGTT GYDYRLNMAQ AAAAAAVPGS QWWYSAANQG QVDANTAAQL QHQQQQQQQQ QQQQQQQHQQ QQQMQQQQQQ QNVINSASPM SRVKADAKPR GRMTAYAYFV QTCREEHKKK HPDETVIFAE FSRKCAERWK TMVDKEKKRF HEMAEKDKQR YEAEMQNYVP PKGAVVGRGK KRKQIKDPNA PKRSLSAFFW FCNDERNKVK ALNPEFGVGD IAKELGRKWS DVDPEVKQKY ESMAERDKAR YEREMTEYKT SGKIAMSAPS MQASMQAQAQ KAALLAAAAQ QQHQQLEEQH DDDDGDGDDD ENQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds preferentially single-stranded DNA and unwinds double-stranded DNA.
Gene References into Functions
  1. involved in the regulation of the kni gap gene PMID: 12820172
  2. binding of DSP1 to Polycomb response elements is important for the recruitment of Polycomb group proteins PMID: 15791260
  3. affects gene expression regulation in Drosophila. PMID: 16115448
  4. DSP1 and corto proteins co-localize at 91 sites on polytene chromosomes and co-immunoprecipitate in embryos. PMID: 16613610
  5. Molecular modeling was used to compare the tertiary structure of Dsp1 with two other Drosophila proteins. PMID: 17785540
  6. While Dorsal seems to be nuclearly localized in ventral nuclei, it is actively shuttling into and out of all nuclei, including nuclei on the dorsal side. PMID: 17978003

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Subcellular Location
Nucleus. Chromosome.
Protein Families
HMGB family
Database Links

KEGG: dme:Dmel_CG12223

STRING: 7227.FBpp0288398

UniGene: Dm.2879

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