Recombinant Drosophila melanogaster Histone RNA hairpin-binding protein (Slbp)

Code CSB-YP896064DLU
MSDS
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Source Yeast
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Code CSB-EP896064DLU
MSDS
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Source E.coli
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Code CSB-EP896064DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896064DLU
MSDS
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Source Baculovirus
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Code CSB-MP896064DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slbp
Uniprot No.
Alternative Names
Slbp; CG11886; Histone RNA hairpin-binding protein; Histone stem-loop-binding protein
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-276
Target Protein Sequence
MLCEDQHMSV ENTPQKGSGS LNSSASSISI DVKPTMQSWA QEVRAEFGHS DEASSSLNSS AASCGSLAKK ETADGNLESK DGEGREMAFE FLDGVNEVKF ERLVKEEKLK TPYKRRHSFT PPSNENSRSN SPNSSNSSAN GDAAAPKGGN NPHSRNSKKS GNFRAHKEEK RVRHNSYTSS TSSSSSYTEA DPAILSRRQK QIDYGKNTAA YERYVEMVPK DERTRDHPRT PNKYGKYSRR AFDGLVKIWR KSLHIYDPPT QARDTAKDSN SDSDSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in histone pre-mRNA 3' processing and couples histone mRNA production with the cell cycle. Both maternal and zygotic proteins play an essential and vital function for development.
Gene References into Functions
  1. Although the C-terminal tail of dSLBP does not contact the RNA, phosphorylation of the tail promotes SLBP conformations competent for RNA binding and thereby appears to reduce the entropic penalty for the association. PMID: 25002523
  2. The nuclear magnetic resonance and kinetic studies presented here provide a framework for understanding how SLBP recognizes histone mRNA and highlight possible structural roles of phosphorylation and proline isomerization in RNA binding proteins PMID: 22439849
  3. Slbp mutants display genomic instability, including loss of heterozygosity (LOH), increased presence of chromosome breaks, tetraploidy, and changes in position effect variegation (PEV). PMID: 19997601
  4. Developmental control of histone mRNA and dSLBP synthesis during Drosophila embryogenesis and the role of dSLBP in histone mRNA 3' end processing in vivo. PMID: 11884612
  5. Phosphorylation of T120 and T230 site of Stem-loop binding protein (SLBP) differentially affects the ability of the protein to restore viability and histone mRNA processing. PMID: 14999087
  6. characterize the structural and dynamic features of the N-terminal domain of SLBP from Drosophila PMID: 15260482
  7. electrostatic effect of protein phosphorylation can be partially mimicked by a mutant form of SLBP wherein four C-terminal serines are replaced with glutamic acids PMID: 15260483
  8. Removal of the phosphoryl group from T230 by either dephosphorylation or mutation results in a 7-fold reduction in the affinity of SLBP for the stem-loop RNA PMID: 16492733

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Protein Families
SLBP family
Tissue Specificity
In late embryos, expression is restricted to proliferating (CNS and PNS) and endoreplicating (midgut) cell populations.
Database Links

KEGG: dme:Dmel_CG11886

STRING: 7227.FBpp0084778

UniGene: Dm.4801

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