Recombinant Drosophila melanogaster Histone-lysine N-methyltransferase ash1 (ash1), partial

Code CSB-YP895203DLU
MSDS
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Source Yeast
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Code CSB-EP895203DLU
MSDS
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Source E.coli
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Code CSB-EP895203DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895203DLU
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Source Baculovirus
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Code CSB-MP895203DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ash1
Uniprot No.
Alternative Names
ash1; KMT2H; CG8887; Histone-lysine N-methyltransferase ash1; EC 2.1.1.354; Absent small and homeotic disks protein 1; Lysine N-methyltransferase 2H
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. TrxG proteins are generally required to maintain the transcriptionally active state of homeotic genes throughout development. Does not act as a coactivator required for transcriptional activation, but specifically prevents inappropriate Polycomb Group (PcG) silencing of homeotic genes in cells in which they must stay transcriptionally active.
Gene References into Functions
  1. Mrg15 is a subunit of the Ash1 complex, a stimulator of Ash1 enzymatic activity and a critical regulator of the TrxG protein function of Ash1 in Drosophila. PMID: 29158494
  2. Ash1 HMTase activity is activated by MRG15, a subunit of the identified Ash1 complex, and that this complex, rather than the Ash1 protein alone, is the active form of this H3K36 methyltransferase, both in vitro and in vivo. PMID: 29540501
  3. If ASH1 or TRX function is lost or reduced, heterochromatin can spread into these domains creating a sink that diverts heterochromatic proteins from other variegating locations, which then may express a suppressed phenotype. PMID: 27373142
  4. Physical interaction of ASH1 with FSH activates global transcription. PMID: 23442797
  5. loss of ASH1 significantly decreases H3K36 dimethylation, providing further evidence that ASH1 is an H3K36 dimethylase in vivo. PMID: 24004944
  6. implication that ASH1 is required for essentially all H3-K4 methylation that occurs in vivo PMID: 13679578
  7. lysine to arginine substitution of histone H3 at position 36 abolished the methyltransferase activity of Drosophila ASH1, suggesting that K36 is their specific target PMID: 17544230

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily
Tissue Specificity
Expressed throughout development but is present at higher levels during the embryonic and pupal stages than during the larval stages. During the larval stages it accumulates primarily in imaginal disks.
Database Links

KEGG: dme:Dmel_CG8887

STRING: 7227.FBpp0297151

UniGene: Dm.7698

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