Recombinant Drosophila melanogaster Histone-lysine N-methyltransferase pr-set7 (pr-set7), partial

Code CSB-YP896087DLU
MSDS
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Source Yeast
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Code CSB-EP896087DLU
MSDS
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Source E.coli
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Code CSB-EP896087DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896087DLU
MSDS
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Source Baculovirus
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Code CSB-MP896087DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pr-set7
Uniprot No.
Alternative Names
PR-Set7; KMT5A; CG3307Histone-lysine N-methyltransferase PR-Set7; EC 2.1.1.361; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; dSET8
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, possibly by contributing to the maintenance of proper higher-order structure of DNA and chromosome condensation during mitosis.
Gene References into Functions
  1. coincident with the ATR-dependent cell cycle arrest, increased DNA damage is specifically limited to late replicating regions of the Drosophila genome, suggesting that PR-Set7-mediated monomethylation of H4K20 is critical for maintaining the genomic integrity of late replicating domains PMID: 27131378
  2. Results support the hypothesis that methylation of H4 lysine 20 by PR/Set7 maintains silent chromatin, in part, by precluding neighboring acetylation on the H4 tail PMID: 12086618
  3. crystal structure reveals the basis of the specificity of the enzyme for histone H4-K20 PMID: 15933069
Subcellular Location
Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates to chromatin-dense and transcriptionally silent euchromatic regions.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, PR/SET subfamily
Database Links

KEGG: dme:Dmel_CG3307

STRING: 7227.FBpp0082387

UniGene: Dm.36781

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