Recombinant Drosophila melanogaster Homeobox protein B-H2 (B-H2), partial

Code CSB-YP636550DLU
MSDS
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Source Yeast
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Code CSB-EP636550DLU
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Source E.coli
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Code CSB-EP636550DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP636550DLU
MSDS
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Source Baculovirus
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Code CSB-MP636550DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
B-H2
Uniprot No.
Alternative Names
B-H2; BarH2; CG5488Homeobox protein B-H2; Homeobox protein BarH2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
B-H1 and B-H2 are regulated by members of the wg signaling pathway; wg and dpp. B-H1 and B-H2 are coexpressed and functionally required in R1 and R6 receptor cells and primary pigment cells for normal eye development. Coexpression is also required for the fate determination of external sensory organs, formation of notal microchaetae, formation of presutural macrochaetae, antennal development and for distal leg morphogenesis; segmentation and specification of tarsal segments 3-5.
Gene References into Functions
  1. Loss of Bar-H2 from the undifferentiated retinal precursor cells leads to ectopic expression of Prospero and dPax2, two transcription factors essential for cone cell specification, resulting in excess cone cell differentiation. PMID: 24505414
  2. data show that Atonal repression is mediated by the conserved homeobox proteins BarH1 and BarH2 PMID: 14573515
  3. Bar expression in the basal undifferentiated cells is regulated by at least three different pathways, depending on the developmental time and the position in the eye disc PMID: 15496446
  4. describe the restricted expression pattern of the B-H2 homeoprotein within the embryonic ventral nerve cord of Drosophila PMID: 16882006
Subcellular Location
Nucleus.
Protein Families
Antp homeobox family
Tissue Specificity
B-H1 and B-H2 are abundant in the eye-antenna imaginal disk. Expressed in R1 and R6 cells throughout larval stage until 30 hours after puparium formation, at which time expression is seen in the anterior and posterior primary pigment cells. Coexpressed in
Database Links

KEGG: dme:Dmel_CG5488

STRING: 7227.FBpp0074203

UniGene: Dm.2611

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