Recombinant Drosophila melanogaster Homeotic protein antennapedia (Antp)

Code CSB-YP360889DLU
MSDS
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Source Yeast
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Code CSB-EP360889DLU
MSDS
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Source E.coli
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Code CSB-EP360889DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360889DLU
MSDS
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Source Baculovirus
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Code CSB-MP360889DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Antp
Uniprot No.
Alternative Names
Antp; ANTP_DROME; Homeotic protein antennapedia
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-378
Target Protein Sequence
MTMSTNNCES MTSYFTNSYM GADMHHGHYP GNGVTDLDAQ QMHHYSQNAN HQGNMPYPRF PPYDRMPYYN GQGMDQQQQH QVYSRPDSPS SQVGGVMPQA QTNGQLGVPQ QQQQQQQQPS QNQQQQQAQQ APQQLQQQLP QVTQQVTHPQ QQQQQPVVYA SCKLQAAVGG LGMVPEGGSP PLVDQMSGHH MNAQMTLPHH MGHPQAQLGY TDVGVPDVTE VHQNHHNMGM YQQQSGVPPV GAPPQGMMHQ GQGPPQMHQG HPGQHTPPSQ NPNSQSSGMP SPLYPWMRSQ FGKCQERKRG RQTYTRYQTL ELEKEFHFNR YLTRRRRIEI AHALCLTERQ IKIWFQNRRM KWKKENKTKG EPGSGGEGDE ITPPNSPQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis.
Gene References into Functions
  1. The Scr promoter interacts with distinct elements in the ftz-Antp interval. PMID: 26391952
  2. High levels of Antp promote the identity of motoneurons that target the distal regions of the femur and tibia. PMID: 23536569
  3. Functional synthetic Antennapedia genes and the YPWM motif play dual roles in transcriptional activation and repression. PMID: 21712439
  4. The function of Antennapedia in the leg is not to repress homothorax, as has been suggested, but to directly repress spineless and other antennal genes that would otherwise be activated within this ring. PMID: 20727877
  5. N-tail of Antp also improves the binding affinity for DNA, with a major contribution by the released waters. PMID: 19919153
  6. Mutants affect cuticular hydrocarbons in females only, with increases in dienes and decrease in monoenes. PMID: 11865713
  7. Binding isotherms of the Antp peptide penetratin, which corresponds to the third helix of the Antp homeodomain, demonstrate that the affinity of penetratin for lipid membranes is largely governed by nonspecific electrostatic interactions. PMID: 12525169
  8. The Antennapedia-derived cell-penetating peptide penetratin can translocate through lipid bilayers by a potential- and composition-dependent pathway that causes only minimal perturbation to the overall integrity and barrier function of the bilayer. PMID: 14636045
  9. Temporal and spatial regulation of the homeotic selector gene Antennapedia in the leg disc is necessary for normal leg development in Drosophila. PMID: 15013806
  10. Trp48 and Trp56 of Antp(43-58) insert into the hydrophobic core of the membrane bilayer and induce a change in the orientation of the negatively charged dimyristoylphosphatidylglycerol (DMPG)headgroups. PMID: 16042388
  11. the posterior signalling centre is specified early in the embryo by the homeotic gene Antennapedia (Antp) and expresses the signalling molecule Hedgehog PMID: 17361183
  12. Temporal and dosage relationships are more subtle than suspected and must be part of a robust understanding of molecular network behaviour involved in determining appendage identity in Drosophila melanogaster. PMID: 17968139
  13. BIP2 binds with the YPWM motif of ANTP in vitro, as well as in vivo, transforming eye to wing tissue. PMID: 18367556
  14. homeodomain supports multiple molecular regulatory functions in addition to protein-DNA and protein-RNA interactions PMID: 18755899
  15. Antagonistic roles for ubx and Antp in regulating segment-specific apoptosis of differentiated motoneurons in the Drosophila embryionic central nervous system are reported. PMID: 18799545

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Subcellular Location
Nucleus.
Protein Families
Antp homeobox family
Database Links

KEGG: dme:Dmel_CG1028

STRING: 7227.FBpp0081161

UniGene: Dm.3803

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