Antp Antibody

Code CSB-PA360889XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Antp Polyclonal antibody
Uniprot No.
Target Names
Antp
Alternative Names
Antp antibody; ANTP_DROME antibody; Homeotic protein antennapedia antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Antp protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis.
Gene References into Functions
  1. The Scr promoter interacts with distinct elements in the ftz-Antp interval. PMID: 26391952
  2. High levels of Antp promote the identity of motoneurons that target the distal regions of the femur and tibia. PMID: 23536569
  3. Functional synthetic Antennapedia genes and the YPWM motif play dual roles in transcriptional activation and repression. PMID: 21712439
  4. The function of Antennapedia in the leg is not to repress homothorax, as has been suggested, but to directly repress spineless and other antennal genes that would otherwise be activated within this ring. PMID: 20727877
  5. N-tail of Antp also improves the binding affinity for DNA, with a major contribution by the released waters. PMID: 19919153
  6. Mutants affect cuticular hydrocarbons in females only, with increases in dienes and decrease in monoenes. PMID: 11865713
  7. Binding isotherms of the Antp peptide penetratin, which corresponds to the third helix of the Antp homeodomain, demonstrate that the affinity of penetratin for lipid membranes is largely governed by nonspecific electrostatic interactions. PMID: 12525169
  8. The Antennapedia-derived cell-penetating peptide penetratin can translocate through lipid bilayers by a potential- and composition-dependent pathway that causes only minimal perturbation to the overall integrity and barrier function of the bilayer. PMID: 14636045
  9. Temporal and spatial regulation of the homeotic selector gene Antennapedia in the leg disc is necessary for normal leg development in Drosophila. PMID: 15013806
  10. Trp48 and Trp56 of Antp(43-58) insert into the hydrophobic core of the membrane bilayer and induce a change in the orientation of the negatively charged dimyristoylphosphatidylglycerol (DMPG)headgroups. PMID: 16042388
  11. the posterior signalling centre is specified early in the embryo by the homeotic gene Antennapedia (Antp) and expresses the signalling molecule Hedgehog PMID: 17361183
  12. Temporal and dosage relationships are more subtle than suspected and must be part of a robust understanding of molecular network behaviour involved in determining appendage identity in Drosophila melanogaster. PMID: 17968139
  13. BIP2 binds with the YPWM motif of ANTP in vitro, as well as in vivo, transforming eye to wing tissue. PMID: 18367556
  14. homeodomain supports multiple molecular regulatory functions in addition to protein-DNA and protein-RNA interactions PMID: 18755899
  15. Antagonistic roles for ubx and Antp in regulating segment-specific apoptosis of differentiated motoneurons in the Drosophila embryionic central nervous system are reported. PMID: 18799545

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Subcellular Location
Nucleus.
Protein Families
Antp homeobox family
Database Links

KEGG: dme:Dmel_CG1028

STRING: 7227.FBpp0081161

UniGene: Dm.3803

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