Recombinant Drosophila melanogaster Kinesin heavy chain (Khc), partial

1. Kinesin-1 slides free cytoplasmic microtubules against cortically immobilized microtubules, generating forces that contribute to cytoplasmic streaming and are essential for the refinement of posterior determinants. PMID: 27512034
2. The results demonstrate that microtubule sliding by kinesin-1 is an essential biological phenomenon required for neuronal morphogenesis and normal nervous system development. PMID: 27512046
3. Tm1C-Khc interaction is specific for the osk localization pathway. PMID: 27802167
4. The authors demonstrate that kinesin-1 recruitment requires the DmTropomyosin1-I/C isoform, an atypical RNA-binding tropomyosin that binds directly to dimerizing oskar 3'UTRs. PMID: 28028052
5. The Kinesin-5 Chemomechanical Cycle Is Dominated by a Two-heads-bound State. PMID: 27402829
6. Tau inhibits the Eg5 kinesin and cell mitosis. PMID: 26822478
7. During the initial stages of axon outgrowth, microtubules display mixed polarity and minus-end-out microtubules push the tip of the axon, consistent with kinesin-1 driving outgrowth by sliding antiparallel microtubules. PMID: 26615019
8. Data show that kinesin-1 spends half of each stepping cycle with one head bound, specifying a structural state for each of two rate-limiting transitions. PMID: 26676576
9. Regenerating neurons contain actively sliding microtubules; this sliding, like sliding during initial neurite outgrowth, is driven by kinesin-1 and is required for axonal regeneration. PMID: 25657321
10. Depletion of Pav-KLP in neurons strongly stimulated kinesin-1-driven sliding of long microtubules and neurite outgrowth, while its ectopic overexpression in the cytoplasm blocked both of these processes. PMID: 25557664
11. kinesin dissociation occurs subsequent to, or concomitant with, phosphate (P(i)) release following ATP hydrolysis PMID: 25197045
12. single-molecule motility from different kinesin-1 and kinesin-2 neck-linker chimeras stepping along microtubules, was studied. PMID: 24739168
13. These results stress that two unexpected highly conserved domains, namely the auto-inhibitory IAK and the auxiliary microtubule-binding motifs, are crucial for transport by Kinesin-1 and that, although not all cargos are conserved, their transport involves the most conserved domains of animal KHCs. PMID: 24257625
14. data implicate Kinesin and Dynein in two distinct and independently regulated mechanisms of moving myonuclei, which together maximize the ability of myonuclei to achieve their proper localizations within the constraints imposed by embryonic development PMID: 24335254
15. A hingeless mutant of kinesin-1, which mimics the active conformation of the motor, does not require ensconsin for transport in S2 cells, suggesting that ensconsin plays a role in relieving autoinhibition of kinesin-1. PMID: 23394833
16. Ectopic expression of Drosophila Khc carrying a human SPG10-associated mutation (N256S) is sufficient to disturb axonal transport and to induce motoneuron disease in Drosophila PMID: 23209432
17. routes to suppression of the neurodegenerative phenotypes caused by kinesin heavy chain mutations PMID: 22714410
18. identification of the microtubule-associated protein ensconsin (Ens)/microtubule-associated protein 7 (MAP7) and kinesin heavy chain (Khc)/Kif5b as essential, evolutionarily conserved regulators of myonuclear positioning in Drosophila and cultured mammalian myotubes PMID: 22425998
19. This study suggested that the role of Khc for neuronal excitability must be considered in the light of its necessity for directed transport in glia. PMID: 22649226
20. Results confirm that KLC contains tetratricopeptide repeat units; a regular array of repeating 34-residue helix-loop-helix monomers. PMID: 22330276
21. behavior of multiple kinesin motors working to overcome a larger load PMID: 21735291
22. analysis of dendritic and axonal phenotypes of kinesin heavy chain (KHC) loss-of-function clones revealed a role for KHC in maintaining polarity of neurons, as well as ensuring proper axonal outgrowth PMID: 21880894
23. role of kinesin heavy chain in the regulation of the apical Crb domain in developing photoreceptors PMID: 21695062
24. study reports the crystal structure of a motor domain dimer in complex with its tail domain at 2.2 angstroms and compares it with a structure of the motor domain alone at 2.7 angstroms PMID: 21836017
25. Kinesin is required for posteriorly localized germ plasm assembly, but not for transport of germ plasm to nuclei. PMID: 21376599
26. The Khc tail is not constitutively required for Kinesin-1 activation, force transduction or linkage to cargo. It might be crucial for more subtle elements of motor control and coordination in the stop-and-go movements of biased saltatory transport. PMID: 21307100
27. Study suggest an important universal function of kinesin-1 heavy chain is to mediate cytoplasmic microtubule-microtubule sliding. PMID: 20566873
28. show that PAT1, an APP-binding protein, interacts with Kinesin-1, functions in the transport of oskar mRNA and Dynein and is required for the efficient motility of KHC along microtubules. PMID: 20630947
29. Self-inhibition of kinesin depends on electrostatic interactions between the motor domain, the tail domain, and a microtubule. PMID: 20446754
30. Results impose constraints on kinesin walking models and suggest a role for rotational freedom in cargo transport. PMID: 19805111
31. data strongly support an inchworm mechanism for the processive, high-duty-ratio movement of kinesin dimers PMID: 11823639
32. Pathway of ADP-stimulated ADP release and dissociation of tethered kinesin from microtubules. Implications for the extent of processivity PMID: 11914091
33. Kinesin may generate anterodorsal forces directly, despite the preponderance of minus ends at the anterior cortex. Alternatively, kinesin I may facilitate cytoplasmic dynein-based anterodorsal forces by repositioning dynein toward microtubule plus ends. PMID: 12225672
34. Data show that kinesin heavy chain can function independently of the light chain in the oocyte, indicating that it associates with its cargoes by a novel mechanism. PMID: 12403717
35. Substitution of Glu164 with alanine in the motor domain results in a dimeric kinesin (E164A) with a dramatic reduction in microtubule-activated steady-state ATPase that is stalled on the microtubule and cannot bind and hydrolyze ATP at the second head. PMID: 12614154
36. the discovery that kinesin limps along the microtubule, taken together with its other nanomechanical properties, implies that it advances by some form of asymmetric hand-over-hand mechanism PMID: 14657506
37. Glutamate 164 of the kinesin heavy chain is shown to be critical for kinesin function, its contribution providing new insights about the motor-microtubule interface and the pathway of communication for processive motility. PMID: 15005614
38. sensitivity of the 16-nm displacements to nucleotide and load raises the possibility that ADP release is a force-producing event of the kinesin cycle PMID: 15257294
39. Drosophila fragile X mental retardation protein associates with both kinesin heavy chain and dynein heavy chain, but not kinesin light chain PMID: 15583137
40. Data show that the plus-end-directed microtubule motor kinesin-1 is required for cytoplasmic streaming and is constitutively capable of driving fast streaming. PMID: 16077093
41. A functional consequence of myosin V's diffusion on microtubules is a significant enhancement of the processive run length of kinesin when both motors are present on the same cargo. PMID: 18347333
42. kinesin-1 gating mechanisms in which one head stimulates the rate of essential processes in the other. PMID: 18702529
43. Hinge 1 domain of kinesin-1 thus facilitates the cooperation of motors by preventing them from impeding each other PMID: 18775962
44. Data suggest that lipid droplets in vivo can simultaneously engage multiple kinesin-1 numbers and that transport properties are largely unaffected by variation in motor number. PMID: 19070579
45. We find that mGFP-labeled kinesin-1 detaches prematurely from microtubules when it encounters obstacles, has a low probability to wait briefly when encountering roadblocks, and can occasionally pass obstacles on the protofilament track. PMID: 19383477
46. Glu426 is involved in stabilization of the microtubule-kinesin complexes when the kinesins are in the most labile, ADP-bound state. PMID: 19622631
47. only a single head is bound to the microtubule between steps at low ATP concentrations; linked head could only rebind the microtubule once ATP had become bound to its partner head PMID: 19693012
48. Kinesin heads take different vertical trajectories during alternate steps, and that the rates for these motions are differentially sensitive to load. PMID: 19751671

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KEGG: dme:Dmel_CG7765

STRING: 7227.FBpp0086328

UniGene: Dm.7076