Recombinant Drosophila melanogaster Peptidoglycan-recognition protein SC2 (PGRP-SC2)

The gene fragment encoding the 21-184aa of the Drosophila melanogaster peptidoglycan-recognition protein SC2 (PGRP-SC2) was co-expressed with the N-terminal 10xHis-tag and C-terminal Myc-tag in the E.coli. The resulting protein is the recombinant Drosophila melanogaster PGRP-SC2 protein. Its purity is greater than 90% determined by SDS-PAGE. Importantly, this recombinant Drosophila melanogaster PGRP-SC2 protein has been cited by a high score-IF paper.

As its name implies, PGRP-SC2 being an amidase degrades bacterial peptidoglycan (PGN) and thus participates in innate immunity. PGRP-SC2 is the homolog of the anti-inflammatory molecules PGLYRP1-4 and is a negative regulator of dampening immune deficiency (IMD)/Relish innate immune signaling. Linlin Guo et al. demonstrated that in the aging intestine of Drosophila, chronic activation of Foxo decreases expression of PGRP-SC2, inhibition of which results in deregulation of Rel/NFκB activity, leading to commensal dysbiosis, stem cell hyperproliferation, and epithelial dysplasia. Overexpression of PGRP-SC2 in enterocytes prevents dysbiosis, promotes tissue homeostasis, and extends lifespan. Yuchen Chen et al. showed that flies bearing malignant yki3SA-gut tumors cause bacterial overload through the inhibition of gut PGRP-SC2 and consequent systemic IMD-NF-κB activation.