Recombinant Drosophila melanogaster Poly (U)-binding-splicing factor half pint (pUf68), partial

Code CSB-YP819437DLU
MSDS
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Source Yeast
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Code CSB-EP819437DLU
MSDS
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Source E.coli
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Code CSB-EP819437DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP819437DLU
MSDS
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Source Baculovirus
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Code CSB-MP819437DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pUf68
Uniprot No.
Alternative Names
hfp; pUf68; CG12085; Poly(U)-binding-splicing factor half pint; Protein half pint; 68 kDa poly(U)-binding-splicing factor; PUF60 homolog
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Splicing factor that regulates oogenesis and controls both mitosis and mRNA localization in the germline by regulating mRNA splicing of a subset of genes within the ovary. Probably acts by regulating the alternative splice site selection of the otu transcript. Also regulates the alternative splicing of eIF4E1 and grk, while it is not involved in the splicing of par-1, sqd and psq.
Gene References into Functions
  1. Provides a critical molecular link between the the wingless pathway and dMyc-regulated cell growth and proliferation. PMID: 14993190
  2. These results suggest that the eIF4E-1,2 gene is regulated by Hfp through a mechanism linked to transcription control and 3' splice site selection, which determines the pattern and translation efficiency of eIF4E-1,2 mRNAs. PMID: 18671938
Subcellular Location
Nucleus.
Protein Families
RRM half pint family
Tissue Specificity
Expressed in all germline cells and within the follicle cell.
Database Links

KEGG: dme:Dmel_CG12085

STRING: 7227.FBpp0072593

UniGene: Dm.7331

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