Recombinant Drosophila melanogaster Polypeptide N-acetylgalactosaminyltransferase 35A (Pgant35A), partial

Code CSB-YP847598DLU
MSDS
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Source Yeast
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Code CSB-EP847598DLU
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Source E.coli
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Code CSB-EP847598DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847598DLU
MSDS
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Source Baculovirus
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Code CSB-MP847598DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pgant35A
Uniprot No.
Alternative Names
Pgant35A; CG7480Polypeptide N-acetylgalactosaminyltransferase 35A; EC 2.4.1.41; Protein l(2)35Aa; Protein-UDP acetylgalactosaminyltransferase 35A; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 35A; pp-GaNTase 35A; dGalNAc-T1
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2. Not involved in glycosylation of erythropoietin (EPO). It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Protein modification by this enzyme might be important for cytokinesis and tube formation during embryogenesis. Together with Pgant3, regulates integrin levels and activity-dependent integrin signaling at the synapse in neurons and muscles.
Gene References into Functions
  1. Drosophila embryogenesis requires functional pgant35A; the presence of this gene product during fly embryogenesis is functionally distinct from other Drosophila GalNAc-transferase isoforms and from the proposed human ortholog GALNT11. PMID: 20422447
  2. essential for viability in Drosophila melanogaster PMID: 11925446
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 2 family, GalNAc-T subfamily
Tissue Specificity
Expressed at high level in ovaries. Expressed at low level in testis. Expressed at higher level in adult females than males. During oogenesis, it is detected in germ cells and follicle epithelia of all developmental stages. Initially expressed during earl
Database Links

KEGG: dme:Dmel_CG7480

STRING: 7227.FBpp0080202

UniGene: Dm.1528

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