Product Details

Purity

>85% (SDS-PAGE)

Target Names

orb2

Uniprot No.

Q9VSR3

Alternative Names

orb2; CG43782; Translational regulator orb2

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

RNA-binding protein involved in translational regulation and required for long-term memory. Required in mushroom body gamma neurons for long-term memory in male courtship. Binds to mRNA 3'-UTRs. In its monomeric form, acts as a translational repressor of genes involved in neuronal growth, synapse formation and protein turnover. In its amyloid-like oligomeric form, acts as a translational activator. The monomeric form reduces poly(A) tail length and destabilizes mRNA while the oligomeric form protects and elongates the poly(A) tail and stabilizes mRNA. Involved in asymmetric cell division in the central nervous system. Plays a role in synapse formation and morphology at neuromuscular junctions by modulating the translation of the tumor suppressor brat. Required for the progression of spermatogenesis through meiosis and for sperm differentiation. During sperm differentiation, required to asymmetrically localize and activate the translation of protein kinase aPKC mRNAs which is necessary for spermatid cyst polarization. Also required during spermatid cyst polarization for localization and translation of its own mRNA.; Required for initial memory acquisition. Following subsequent late dopaminergic pathway activation, recruits isoform B into a complex to activate translation of CaMKII which is required for long-term memory consolidation.

Gene References into Functions

We present here evidences of a novel genetic mechanism to regulate synapse fine tuning during development and propose an equilibrium between Orb2 conformational state and nervous system formation. PMID: 29105522
The N-terminus of Orb2A not only promotes the formation of fibrils, but also forms the fibril core. PMID: 27922050
Data confirm the original hypothesis that the glutamine, histidine-rich (Q/H-rich) domain of Orb2 can bind to transition metals via its His residues. Hence, the Q/H rich domain behaves like other H-rich domains, most of which were shown to be metal binding domains. PMID: 28763009
Study showed that the N-terminus specific to Orb2A can bind to lipid membranes; binding occurs preferentially to curved membranes and requires the membranes to be negatively charged. When binding to lipid membranes, the N-terminus of Orb2A undergoes a transition from a dynamic, intrinsically disordered state to a less dynamic alpha-helix. This lipid membrane interaction can prevent the N-terminus from forming amyloid f... PMID: 28700922
Authors posit that pasilla-mediated processing of unspliced Orb2A mRNA integrates experience and internal state to control Orb2A protein abundance and long-term memory formation. PMID: 28525754
rapidly forms amyloids and its toxic intermediates are extremely transient PMID: 26812143
Data support a model in which monomeric Orb2 keeps target mRNA in a translationally dormant state and experience-dependent conversion to the amyloidogenic state activates translation, resulting in persistent alteration of synaptic activity and stabilization of memory. PMID: 26638074
we present evidence that, during learning, the translational regulator Orb2A tags specific synapses of mushroom body neurons for later consolidation. Consolidation involves the subsequent recruitment of Orb2B and the activity-dependent synthesis of CaMKII PMID: 26095367
analysis of a partial loss of function orb2 mutant suggests that the orb2 differentiation phenotypes are independent of the earlier arrest in meiosis PMID: 23209437
This study demonistrated that Drosophila CPEB Orb2A mediates memory independent of Its RNA-binding domain. PMID: 23083740

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Subcellular Location

Perikaryon. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Cytoplasm. Cytoplasm, perinuclear region.; [Isoform A]: Cell junction, synapse.; [Isoform B]: Perikaryon. Cell projection, axon. Cell junction, synapse.

Tissue Specificity

Broadly expressed throughout the nervous system of embryo, larva and adult including the ventral nerve cord and brain (at protein level). In early embryos, deposited maternally and distributed uniformly throughout the embryo until the extended germband st

Database Links

KEGG: dme:Dmel_CG43782

STRING: 7227.FBpp0303905

UniGene: Dm.4024

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Recombinant Drosophila melanogaster Probable RNA-binding protein orb2 (orb2), partial

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