Recombinant Drosophila melanogaster Probable lysine-specific demethylase 4A (Kdm4A)

Code CSB-YP894170DLU
MSDS
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Source Yeast
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Code CSB-EP894170DLU
MSDS
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Source E.coli
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Code CSB-EP894170DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894170DLU
MSDS
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Source Baculovirus
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Code CSB-MP894170DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Kdm4A
Uniprot No.
Alternative Names
Kdm4A; CG15835; Probable lysine-specific demethylase 4A; EC 1.14.11.-; Probable JmjC domain-containing histone demethylation protein 3A
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-495
Target Protein Sequence
MSTRSSFADE EQNKVPRIMT FRPSYEEFQN FSAYIEYIES RGAHLAGLAK IQPPAEWVPR KSGYDIDNIN MTIPAPICQV VTGAHGVYQQ INIQQRRQMT LRQFMEKANS ELHQTPRHFD YDDLERKYWK NITYISPLYA ADVKGSLSDE DLDVWNIGRL DTILNLVNTD YNIIIDGVNT AYLYFGMWKS SFAWHTEDMD LYSINYLHFG APKTWYAIPP AYGRRLEKLA NETFSENYQE CNAYLRHKMT MISPKVLRQH NIPYNKITQE AGEIMITFPF GYHAGFNHGF NGAESTNFAS KRWIEYGKRA SICRCRSDMV KISMETFVRR FQPERYDNWL KGQDMGCHPE EPGKICAAAP PTLNEYEKQE NLRAAKSEEE SPQKRGCSLA GNGCERNAES AEDVDDKASV SSYSSCRQLQ PVVKLRKLPT IASVPEPSSA PKRYDFNTEA VVRVKRLWNE LPCPDRGANL LTNGVVKNTK RMRFQTKVLT LDDED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate.
Gene References into Functions
  1. dKDM4A, in addition to its previously characterized role in euchromatin, utilizes both enzymatic and structural mechanisms to regulate heterochromatin organization and functions. PMID: 28743002
  2. Jra recruits the HP1a/KDM4A complex to its gene body region upon osmotic stress to reduce H3K36 methylation levels and disrupt H3K36 methylation-dependent histone deacetylation PMID: 25945750
  3. Appropriate expression levels for some dKDM4A-regulated genes rely on the demethylase activity of dKDM4A, whereas others do not. PMID: 23195220
  4. These results suggest a function of HP1a in transcription facilitating H3K36 demethylation at transcribed and/or heterochromatin regions by dKDM4A (CG15835). PMID: 19061644
  5. these results have implications for the role of the epigenetic regulator Dmel\Kdm4A in the control of genes involved in life span and male-specific sex determination in the fly. PMID: 19786080

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Subcellular Location
Nucleus.
Protein Families
JHDM3 histone demethylase family
Database Links

KEGG: dme:Dmel_CG15835

STRING: 7227.FBpp0087961

UniGene: Dm.4139

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