Recombinant Drosophila melanogaster Profilin (chic)

Code CSB-YP333112DLU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP333112DLU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP333112DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP333112DLU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP333112DLU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
chic
Uniprot No.
Alternative Names
chic; chi; CG9553; Profilin; Protein chickadee
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-126
Target Protein Sequence
MSWQDYVDNQ LLASQCVTKA CIAGHDGNIW AQSSGFEVTK EELSKLISGF DQQDGLTSNG VTLAGQRYIY LSGTDRVVRA KLGRSGVHCM KTTQAVIVSI YEDPVQPQQA ASVVEKLGDY LITCGY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. This profilin is required for intercellular cytoplasm transport during Drosophila oogenesis.
Gene References into Functions
  1. Insulin/Insulin-like growth factor signaling specifically activates the expression of the actin regulator chickadee, the Drosophila homolog of Profilin, which is essential for promoting the formation of actin extensions and migration through the egg chamber.and migration through the egg chamber. PMID: 29980565
  2. Chic regulates interactions across the somatic permeability barrier around the germline that is essential for Drosophila spermatogenesis. PMID: 25503408
  3. chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain germline stem cells, possibly facilitating localization or maintenance of E-cadherin to the germline stem cell-hub cell interface. PMID: 24346697
  4. Data show that chic-deficient larvae fail to form a robust actin cable along the wound edge and also fail to form normal filopodial and lamellipodial extensions into the wound gap. PMID: 22976306
  5. D-SCAR and profilin were identified as important regulators of phagocytosis in Drosophila PMID: 12919849
  6. FMRP1 binds the mRNA of the Drosophila profilin and negatively regulates Profilin protein expression PMID: 15964283

Show More

Hide All

Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Profilin family
Tissue Specificity
Expressed in ovary and head.
Database Links

KEGG: dme:Dmel_CG9553

STRING: 7227.FBpp0089196

UniGene: Dm.4775

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1