Recombinant Drosophila melanogaster Protein bag-of-marbles (bam)

Code CSB-YP329340DLU
MSDS
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Source Yeast
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Code CSB-EP329340DLU
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Source E.coli
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Code CSB-EP329340DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329340DLU
MSDS
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Source Baculovirus
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Code CSB-MP329340DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bam
Uniprot No.
Alternative Names
bam; CG10422Protein bag-of-marbles
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-442
Target Protein Sequence
MLNARDVCPE GNDDQQLDHN FKQMEEHLAL MVEGNENEDP RKATCEYEDT NEDGATCTSG VLSEIQENFG RLRLCDVTAP LLEFHGLDCL QQIQKRSRHF AFDGSPAKKS RSGGVLVTGP KQKQLQKENV WNRKSKGSAS ADNIEKLPIT IEKLHMIGLH GDCLEHNAVL RLMNLFRSLH DHLTADLGFS RQNSMPSDYL FDMPVKSTMP KSLNVRYQLQ VLCTKVERFL VQQRRTLEAN RHFDFEKYDE CDKLLKGFAS YLDNFKLLLK PKMRNRNGNS GSNADKFHTQ RMERLLIGLR DWIKAAHLSV HVFNWEMDLE HRYSGAMTES HKSLNERAIL LSGAELRAAE ARGISAEDLF IAQRYKLGGP IYCVLEQHEF LSALIANPET YFPPSVVAIC GPQKLGAVSM EQPSASEEEF EETEEVPSSP PRHTGRVPRF RS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required to initiate both male and female gametogenesis. May regulate cystoblast cell divisions.
Gene References into Functions
  1. Adaptive evolution of bam has been driven at least in part by the long-term interactions between Drosophila species and Wolbachia. PMID: 26291077
  2. Our in vivo and in vitro evidence illustrate that Tut acts with Bam, Bgcn to accurately coordinate proliferation and differentiation in Drosophila germline stem cell lineage. PMID: 25412508
  3. Maintenance of female sexual identity requires the concerted actions of Sxl and bam. PMID: 25758221
  4. We show that bam repression is maintained in ote(-/-) germline stem cells and that germ cell loss persists in ote(-/-), bam(-/-) mutants. PMID: 23806619
  5. Thus, germ cells progress through distinct, sequential regulatory states, from Mei-P26 on/Bam off to Bam on/Mei-P26 off. PMID: 23122292
  6. Although Bam is important for initiating germline stem cell differentiation, its downregulation is crucial for proper spermatid terminal differentiation. PMID: 23154414
  7. Bam/Bgcn complex plays a pivotal role in promoting stem cell differentiation.[Review] PMID: 22127253
  8. the switch from proliferation to terminal differentiation is triggered by accumulation of Bam protein to a critical threshold in transit amplifying cells PMID: 20018708
  9. Repression of bag-of-marbles (bam) expression in male germline stem cells is important for male germline stem cell survival. PMID: 15238523
  10. Results describe the expression and functional analysis of Gm114, a putative mammalian ortholog of Drosophila bam. PMID: 18423593
  11. BAM functions as a translation repressor by interfering with translation initiation. PMID: 19556547

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Tissue Specificity
In cystoblasts and/or very early cystocytes in ovary and testis.
Database Links
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