Recombinant Drosophila melanogaster Protein cortex (cort)

Code CSB-YP853335DLU
MSDS
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Source Yeast
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Code CSB-EP853335DLU
MSDS
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Source E.coli
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Code CSB-EP853335DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853335DLU
MSDS
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Source Baculovirus
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Code CSB-MP853335DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cort
Uniprot No.
Alternative Names
cort; CG11330; Protein cortex
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-483
Target Protein Sequence
MFVEMSVCDS PNKNSKLDKK SLTPFKKVRR KNWKQEAAYK SDTSKGQEVS YVGERFIPNR FERENIEFNL KYIGKRKERD ILETGVTLTA SYWRQSGFIS NINRTFGIGE RRLFQFSSQQ GTRSRVVDND SADSDWPCNP RARPYAIQNA THEMPGICSP VDYNMMDWSS GGMVAMSSGQ DVMLWRNLDE STMVFSVESP TSLKYSPDGK HLAIGCMDRN YPVLDLWEVR SPTEFLVSYR KLFFKSMGYI SCIEWSHDGK EVICGTQCGV IIVLAMPTLN TLMQLREHRH TVKKMKFAPT HKYFASSDTD GKIFIFDAVL KVRLLKLDGR SIVFDWHPWT GEDLAVAERS PASIFIFNIP RRQFVASYRR RDDRIVIKTL TYSKITGELL VNVIRRDDAD LAVCEILVLA SLNRVVDLMS HQDRGTLFLM WNPDGTKIAT GGLDDTFSLW NFFPTYKREA ILRKQEQKAK DKCSSLSLYK GIR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Female meiosis-specific activator of the anaphase promoting complex/cyclosome (APC/C). Required for the completion of meiosis in oocytes. Activates the ubiquitin ligase activity and substrate specificity of APC/C and triggers the sequential degradation of mitotic cyclins in meiosis. Promotes the ubiquitination and degradation of CycA early in meiosis I and the degradation of CycB and CycB3 after egg activation. Promotes degradation of mtrm at the oocyte-to-embryo transition.
Gene References into Functions
  1. These data indicate APC(Cort) ubiquitylates Mtrm at the oocyte-to-embryo transition, thus preventing excessive inhibition of Polo kinase activity due to Mtrm's presence. PMID: 24019759
  2. Cks30A interacts with Cdk1 to target cyclin A for destruction in the female germline, possibly through the activation of a novel germline specific CDC20 protein, Cortex PMID: 16138012
  3. Cyclin B associates with spindle microtubules throughout meiosis I and meiosis II, and dissociates from the meiotic spindle in anaphase II. Fzy and Cort are required for this loss of cyclin B from the meiotic spindle. PMID: 17251266
  4. CORT physically associates with core subunits of the APC/C in ovaries. APC/C(CORT) targets Cyclin A for degradation prior to the metaphase I arrest, while Cyclins B and B3 are not targeted until after egg activation. PMID: 18020708
Subcellular Location
Cytoplasm.
Protein Families
WD repeat CORT family
Tissue Specificity
Highly expressed in ovaries. Expressed in nurse cells from stage 6 to stage 12 egg chambers. As oocyte matures, it is transferred from the nurse cells to the oocyte.
Database Links
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