Recombinant Drosophila melanogaster Protein diaphanous (dia), partial

Code CSB-YP342172DLU
MSDS
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Source Yeast
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Code CSB-EP342172DLU
MSDS
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Source E.coli
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Code CSB-EP342172DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP342172DLU
MSDS
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Source Baculovirus
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Code CSB-MP342172DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dia
Uniprot No.
Alternative Names
dia; CG1768Protein diaphanous
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for cytokinesis in both mitosis and meiosis. Has a role in actin cytoskeleton organization and is essential for many, if not all, actin-mediated events involving membrane invagination. May serve as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle. Possible component of the contractile ring or may control its function.
Gene References into Functions
  1. Dia plays two roles during invasive podosome formation at the fusion site: it dictates the level of linear F-actin polymerization, and it is required for appropriate branched actin polymerization via localization of SCAR and WASp. PMID: 26295716
  2. Dia apical targeting also depends on binding to Rho1, which is critical for activation-induced conformational change, as well as physically anchoring Dia to the apical membrane PMID: 23853710
  3. EnaEVH1 binds Dia directly and inhibits DiaFH1FH2-mediated nucleation in vitro PMID: 24576424
  4. The F-BAR protein Cip4/Toca-1 antagonizes the formin Diaphanous in membrane stabilization and compartmentalization. PMID: 23424199
  5. Drosophila and vertebrate APCs exhibit highly related activities and mechanisms and directly collaborate with formins such as diaphanous. PMID: 23558679
  6. Data show that flies expressing mutant diaphanous exhibit significantly reduced sound-evoked potentials (SEPs). PMID: 20624953
  7. Dia and ena work together in a complex and nonadditive way, with the ratio between active Dia and Ena being one factor that modulates the balance between filopodia and lamellipodia. PMID: 19846663
  8. RhoGEF2 and dia control position, shape and stability of the forming furrow canal by spatially restricted assembly of actin filaments required for the proper infolding of the plasma membrane PMID: 15689371
  9. role in regulating myosin levels and activity at adherens junctions during cell shape change, suggesting that Diaphanous helps coordinate adhesion and contractility of the underlying actomyosin ring PMID: 18256194
  10. DRhoGEF2 acts through Rho1 to regulate acto-myosin constriction but not Diaphanous-mediated F-actin nucleation during segmental groove morphogenesis. PMID: 18287521
  11. A model is proposed in which Diaphanous links receptor tyrosine phosphatase signaling at the plasma membrane to growth-dependent modulation of the synaptic actin and microtubule cytoskeletons. PMID: 18971454
  12. APC2 and DIA function in a novel complex that affects actin directly, rather than through an effect on microtubules PMID: 19279137
  13. Secretory vesicles are targeted to the apical membrane by MyoV-based transport, along polarized actin filaments nucleated by Dia. PMID: 19531358

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Subcellular Location
Cytoplasm, cytoskeleton. Cleavage furrow. Note=Localizes to the site where the metaphase furrow is anticipated to form, to the growing tip of cellularization furrows, and to contractile rings.
Protein Families
Formin homology family, Diaphanous subfamily
Database Links

KEGG: dme:Dmel_CG1768

STRING: 7227.FBpp0297103

UniGene: Dm.4235

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