Recombinant Drosophila melanogaster Protein king tubby (king-tubby)

Code CSB-YP774782DLU
MSDS
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Source Yeast
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Code CSB-EP774782DLU
MSDS
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Source E.coli
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Code CSB-EP774782DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP774782DLU
MSDS
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Source Baculovirus
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Code CSB-MP774782DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ktub
Uniprot No.
Alternative Names
ktub; Tulp; CG9398; Protein king tubby
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-460
Target Protein Sequence
MSGINSRNQK MEQQRQLMEA YIRQKRASPG MVQASDLQIN RPMSGMRSNS RELHAYDGPM QFISSPQNPD QILTNGSPGG INPVAMNTSR NHSNNMRSLS TINQEADLIE EISSHELEDE ESSPVTVIEQ HQQSASHSAN STQSQKPRAR QHSFSDNLDE DDYTNRNVAG AAPVRPAGMA SSPYKDATLD GSSNGTGNGT GGESEGDVIG NIDQFVMQPA PQGVLYKCRI TRDRKGMDRG LFPIYYLHLE RDYGKKIFLL GGRKRKKSKT SNYIVSCDPT DLSRNADGFC GKLRSNVFGT SFTVFDNGNK ESTESPRLDL AVIIYDTNIL GFKGPRNMTV ILPGMTEDDQ RVKISSADPK QQGILDLWKM KNMDNIVELH NKTPVWNDET QSYVLNFHGR VTQASVKNFQ LVHDSDPEYI VMQFGRTSED VFTMDYRYPL CAMQAFAIAL SSFDGKIACE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions in regulating protein trafficking, retinal maintenance and lipid storage. Protects photoreceptor cells R1 to R6 against light-induced retinal degeneration by stimulating norpA-mediated endocytosis of the rhodopsin ninaE (Rh1). In the auditory receptor neurons, functions as a cilia trafficking regulator of various transient receptor potential (TRP) channel components including iav and nompC. Likely to deliver pre-ciliary vesicles containing membrane proteins such as iav and nompC to the intraflagellar transport complex (IFT) at the cilia base. Plays a role in the inhibition of fat storage.
Gene References into Functions
  1. This is the first demonstration that dTULP regulates TRP channel localization in cilia, and suggests that dTULP is a protein that regulates ciliary neurosensory functions. PMID: 24068974
  2. The results in this study delimit the novel function of Ktub in Rh1 endocytosis and retinal degeneration PMID: 23228091
  3. TULP is principally expressed in the embryonic central and peripheral nervous systems. PMID: 12204260
Subcellular Location
Cytoplasm. Nucleus. Cell projection, cilium membrane; Peripheral membrane protein.
Protein Families
TUB family
Tissue Specificity
Detected in sensory neurons which have a ciliary structure such as the chordotonal neurons, Orco-expressing olfactory receptor neurons, labellar gustatory receptor neurons and in the femoral chordotonal organ (at protein level). In the chordotonal neurons
Database Links

KEGG: dme:Dmel_CG9398

STRING: 7227.FBpp0088961

UniGene: Dm.3934

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