Recombinant Drosophila melanogaster Protein melted (melt), partial

Code CSB-YP892889DLU
MSDS
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Source Yeast
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Code CSB-EP892889DLU
MSDS
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Source E.coli
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Code CSB-EP892889DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892889DLU
MSDS
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Source Baculovirus
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Code CSB-MP892889DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
melt
Uniprot No.
Alternative Names
melt; CG8624; Protein melted
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Participates in fat metabolism regulation by recruiting FOXO and the TSC1-TSC2 complex to the cell membrane, which positively regulates TOR activity and negatively regulates the expression of FOXO target genes. Involved in R8 photoreceptor subtype specification. During early embryonic development, may be required for ectodermal patterning.
Gene References into Functions
  1. Melted as a new modulator of the insulin/PI3K signaling pathway which controls both tissue growth and metabolism PMID: 16054033
  2. Here, we show that warts, encoding large tumor suppressor (Lats) and melted encoding a PH-domain protein, play opposite roles in defining the yR 8 or pR8 fates in Drosophila ommatidia photoreceptors. PMID: 16143107
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
MELT/VEPH family
Database Links

KEGG: dme:Dmel_CG8624

STRING: 7227.FBpp0291254

UniGene: Dm.9528

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