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Recombinant Drosophila melanogaster Protein tiptop (tio), partial

Code CSB-YP890627DLU
MSDS
MSDS Datasheet
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Source Yeast
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Code CSB-EP890627DLU
MSDS
MSDS Datasheet
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Source E.coli
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Code CSB-EP890627DLU-B
MSDS
MSDS Datasheet
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890627DLU
MSDS
MSDS Datasheet
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Source Baculovirus
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Code CSB-MP890627DLU
MSDS
MSDS Datasheet
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tio
Uniprot No.
Q9U3V5
Alternative Names
tio; CG12630; Protein tiptop
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Protein FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Related Products

tio Antibodies

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Target Background

Function
Tiptop (tio) and teashirt (tsh) have, on the whole, common activities. Tio and tsh repress each other's expression and tsh has a crucial role for trunk patterning that is in part masked by ectopic expression of tiptop. Both genes share a common activity required for the activation of Ser and svb and the maintenance of en and wg.
Gene References into Functions
  1. Tio has four such Zinc Finger domains Tio bind to C-terminal Binding Protein (CtBP) and that this interaction is important for promoting both cell proliferation and eye development. PMID: 22019301
  2. Teashirt and Tiptop repress each other's expression PMID: 15936749
  3. Data show that tiptop appears to be a more effective inducer of retinal formation than teashirt. PMID: 19347955
Subcellular Location
Nucleus.
Protein Families
Teashirt C2H2-type zinc-finger protein family
Tissue Specificity
Expression in the Malpighian tubules (MTs) and stomatogastric nervous system starts at embryonic stage 10. At stage 11, expression in the head domain is initiated in the clypeolabrum in two bilaterally symmetric clusters of cells. At stage 12, expression
Database Links

KEGG: dme:Dmel_CG12630

UniGene: Dm.19461

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