Recombinant Drosophila melanogaster Protein twisted gastrulation (tsg)

Code CSB-YP344956DLU
MSDS
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Source Yeast
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Code CSB-EP344956DLU
MSDS
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Source E.coli
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Code CSB-EP344956DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344956DLU
MSDS
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Source Baculovirus
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Code CSB-MP344956DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tsg
Uniprot No.
Alternative Names
tsg; CG1502; Protein twisted gastrulation
Species
Drosophila melanogaster (Fruit fly)
Expression Region
24-249
Target Protein Sequence
DGCNEVV CGSVVSKCLI TQSCQCKLND CHCCKDCLNC LGELYIECCG CLDMCPKHKD VLPSLTPRSE IGDIEGVPEL FDTLTAEDDE GWSTIRFSMR AGFKQRVQGG ASGDAGNGNG NGNAGSAGVT LCTVIYVNSC IRANKCRQQC ESMGASSYRW FHDGCCECVG ENCLNYGINE SRCRGCPEDQ DQLLTADTVP AEAEQDLERF FGNEEIEDEW GYGEEDEFS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in dorsal-ventral patterning. Required for specification of a narrow strip of dorsal midline cells that will give rise to the amnioserosa, but not for specification of dorsal ectoderm cells. Inhibits BMP signaling; enhances the binding of sog to dpp, thus enhancing the antagonistic activity of sog.
Gene References into Functions
  1. Robustness of the BMP morphogen gradient in Drosophila embryonic patterning PMID: 12239569
  2. Data suggest that decapentaplegic (Dpp)concentration dependence, together with movement of Dpp by Sog and Tsg proteins, can explain how Tld activity is confined to the dorsal-most region of the embryo. PMID: 12925593
Subcellular Location
Secreted.
Protein Families
Twisted gastrulation protein family
Tissue Specificity
First appears in stage 4 embryos, expressed in two domains: a broad mid-dorsal saddle and an anterior cap, expression between the domains is continuous across the dorsal midline. At stage 5, expression is refined into 4 graded stripes in the mid-dorsal re
Database Links

KEGG: dme:Dmel_CG1502

STRING: 7227.FBpp0073426

UniGene: Dm.27553

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