Recombinant Drosophila melanogaster Protein wech(wech) ,partial

Code CSB-YP893708DLU
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Source Yeast
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Code CSB-EP893708DLU
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Source E.coli
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Code CSB-EP893708DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893708DLU
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Source Baculovirus
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Code CSB-MP893708DLU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wech
Uniprot No. Q9V4M2
Alternative Names wech; dpld; CG42396Protein wech; Protein dappled
Species Drosophila melanogaster (Fruit fly)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Vital for larval development. Plays a role in tumor formation. A crucial component for the physical link between integrins and the cytoskeleton in the epidermal muscle attachment sites.
Gene References into Functions
  1. Data show taht dpld seems to be miRNA regulated, suggesting that miRNAs represent an ancient mechanism of LIN-41 regulation. PMID: 18069688
  2. Data suggest that Wech proteins are crucial and evolutionarily conserved regulators of the integrin-cytoskeleton link. PMID: 18327251
Tissue Specificity Expressed ubiquitously in all epithelial cells during early stages of embryogenesis. Specifically expressed at epidermal muscle attachment site.
Database Links

KEGG: dme:Dmel_CG42396

STRING: 7227.FBpp0289359

UniGene: Dm.1824

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Address
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
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