Recombinant Drosophila melanogaster Putative proteasome inhibitor (CG8979)

Code CSB-YP892687DLU
MSDS
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Source Yeast
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Code CSB-EP892687DLU
MSDS
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Source E.coli
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Code CSB-EP892687DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892687DLU
MSDS
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Source Baculovirus
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Code CSB-MP892687DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PI31
Uniprot No.
Alternative Names
PI31; CG8979; Proteasome inhibitor PI31 subunit
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-270
Target Protein Sequence
MESGSTAKTG DFFYGWDLLY KTVKADVSKK SDLLIALVHF LLTKHYNFRC VGVGDDKTLP EEEGSELLPD SWNDDDTKYS LRYVHDKMLY LLLGHITEGS LLINLLDINT KKVSNICVEP ETLVPEVKGG ITTIMPSASE IVERYRRELL DPVFTGNSRE VTTQTTNSPR PIGSDPDPLR IGEPRRGGSF IPSAFEPRPF GFPDVGRGDL DPLGRGGHGN LFSFPSRPNM GPGPVPRFDP FNPLNPNRPG QGGINPDHMR PPDWNPDYYM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an important role in the control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Enhances 26S proteasome function by promoting its assembly through the interaction with the assembly chaperones PSMD9 and PMSD5. Functions together with ntc to control non-apoptotic caspase activation during sperm individualization. In testis, is required for proper protein degradation and germline cell cycle progression.
Gene References into Functions
  1. Study identified DmPI31 as a binding partner of the F box protein Nutcracker, a component of an SCF ubiquitin ligase (E3) required for caspase activation during sperm differentiation in Drosophila. PMID: 21529711
Subcellular Location
Cytoplasm. Note=In the spermatid, localizes to the actin-based individualization complex.
Protein Families
Proteasome inhibitor PI31 family
Tissue Specificity
In testis, expressed in germline stem cells and primary spermatocytes (at protein level).
Database Links

KEGG: dme:Dmel_CG8979

STRING: 7227.FBpp0301842

UniGene: Dm.4218

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