Recombinant Drosophila melanogaster RNA-binding protein lark (lark)

Code CSB-YP835997DLU
MSDS
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Source Yeast
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Code CSB-EP835997DLU
MSDS
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Source E.coli
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Code CSB-EP835997DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835997DLU
MSDS
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Source Baculovirus
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Code CSB-MP835997DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lark
Uniprot No.
Alternative Names
lark; CG8597RNA-binding protein lark
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-352
Target Protein Sequence
MPGAGTFKLF IGNLDEKTQA TELRALFEKY GTVVECDVVK NYGFVHMETE QQGRDAIQNL NGYTLNEFAI KVEAAKSRRA PNTPTTKIFV GNLTDKTRAP EVRELFQKYG TVVECDIVRN YGFVHLDCVG DVQDAIKELN GRVVDGQPLK VQVSTSRVRP KPGMGDPEQC YRCGRSGHWS KECPRLYGSA GGGREPPSPL SAGGYRDRMY GRDPYPPPPP PPPFLRDRIM DGFRDYDYYD RRFEDSRDLY ERRYQTSRMR DFPPPPISRR EPMPLPPTLS GSLRSCSVSR GYDTMFSRRS PPPPRSSNGM SRYGSPTPHG YEDFSRDAFD ERMISSRGMR GPSPPGRRYA PY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential RNA-binding protein. May be required for circadian repression of eclosion. Also essential for nurse cell dumping during oogenesis, the process whereby the cytoplasmic contents of nurse cells are transferred to the oocyte late in it's development.
Gene References into Functions
  1. LARK influences circadian period. PMID: 25211129
  2. LARK is required in the Drosophila circadian system PMID: 22653887
  3. Lark is required for the organization of the actin cytoskeleton and Hu-li Tai Shao localization during oogenesis. PMID: 15452872
  4. RBM4 and Lark are the same protein, are localized in speckles and nucleoli, but can redistribute to perinucleolar clusters, consistent with a novel subnuclear pathway PMID: 16907643
  5. LARK RNA-binding protein selectively regulates the circadian eclosion rhythm by controlling E74 protein expression PMID: 17971870
  6. Data show that increased LARK expression during development dramatically alters the small LNv class of pigment-dispersing factor (PDF)-containing neurons with no obvious effects on the large LNv cells. PMID: 19303442

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Subcellular Location
Cytoplasm. Nucleus. Note=The precise location within neurons varies according to the type of neuron, being cytoplasmic in CCAP neurons and nuclear in the CNS.
Tissue Specificity
Expressed in the CNS and in CCAP neurons of the ventral nervous system (VNS), which control insect ecdysis.
Database Links

KEGG: dme:Dmel_CG8597

STRING: 7227.FBpp0076555

UniGene: Dm.7078

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