Product Details

Purity

>85% (SDS-PAGE)

Target Names

dsh

Uniprot No.

P51140

Alternative Names

dsh; CG18361Segment polarity protein dishevelled

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Required to establish coherent arrays of polarized cells and segments in embryos. Plays a role in wingless (wg) signaling, possibly through the reception of the wg signal by target cells and subsequent redistribution of arm protein in response to that signal in embryos. This signal seems to be required to establish planar cell polarity and identity.

Gene References into Functions

a likely direct interaction between Dishevelled and Fritz, is reported. PMID: 28258110
Exon junction complex (EJC) activity is indispensable for Wg signaling by maintaining an appropriate level of Dsh protein for Wg ligand reception in Drosophila. Genetic and biochemical experiments demonstrate that Dlg1 protein acts independently from its role in cell polarity to protect Dsh protein from lysosomal degradation. PMID: 27536874
Klp64D forms a complex with Dsh and shows functional interaction. PMID: 25063455
The mechanism by which Wingless signalling is able to downregulate Notch is by promoting its ligand-independent traffic to a compartment where it is degraded and that this activity depends on Dishevelled. PMID: 20495361
co-expression experiments reveal that APC displaces Dishevelled from Axin assemblies, promoting degradasome over signalosome formation in the absence of Wnts PMID: 22645652
Mutations that compromise a direct interaction between Dishevelled and Notch reduce the efficacy of repression. PMID: 22736244
while many Ser/Thr and Tyr residues are indeed phosphorylated in vivo, strikingly most of these phosphorylation events are not critical for Dsh function with the exception of DshY473 PMID: 21963539
This result implies that Dishevelled has the potential to act as a "natural" dominant-negative, binding to the polymerization interface of Axin's DIX domain to interfere with its self-assembly PMID: 21245303
Dsh-Lgl interaction contributes to epidermal tissue morphogenesis. PMID: 20736316
Results describe a Frizzled-Dishevelled-NuMA/Mud pathway that orients division from Drosophila to vertebrates. PMID: 21074723
dAbl positively regulates the Fz/Dishevelled (Dsh) PCP pathway without affecting canonical Wnt/Wg-Fz signaling PMID: 20837657
Dishevelled signals through LRP5/6 in human cells and Drosophila embryos. PMID: 20388731
Pk causes dsh to have an asymmetric localization in the cytoplasm of Drosophila. PMID: 12015986
mutational analysis of dishevelled in Drosophila defines novel domains in the dishevelled protein as well as novel suppressing alleles of axin PMID: 12072470
interaction with naked cuticle antagonizing Wnt signaling PMID: 12354775
Genetic interactions and epistasis analysis suggest that frizzled, dishevelled, and fat act together in the long-range propagation of polarity signals in the eye and wing. PMID: 12479810
Data show that Strabismus becomes asymmetrically localised to the proximal edge of cells, then binds directly to Dishevelled and Prickle and is able to recruit them to membranes. PMID: 12756182
Binding of Dishevelled to the ankyrin-repeat protein Diego promotes Frizzled signalling. PMID: 15937478
Lgl associates with Dishevelled, an essential mediator of Wnt signalling, and that Dishevelled regulates the localization of Lgl in Xenopus ectoderm and Drosophila follicular epithelium PMID: 16251968
the interaction of Fz or Dsh with other proteins could modulate the signal transduction via interactions between frizzled and dishevelled PMID: 18065209
These results suggest that direct interaction between Fz and Dsh is stabilized by a pH and charge-dependent interaction of the DEP domain with phospholipids. PMID: 19234454

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Subcellular Location

Cytoplasm. Nucleus. Membrane; Peripheral membrane protein. Note=Associated with the membrane when hyperphosphorylated.

Protein Families

DSH family

Tissue Specificity

Found in egg chambers of the ovary and ubiquitously throughout embryogenesis and in disks. Expression is not seen in salivary glands, muscles or ventral ganglia but is observed in brain lobes.

Database Links

KEGG: dme:Dmel_CG18361

STRING: 7227.FBpp0073310

UniGene: Dm.2927

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Recombinant Drosophila melanogaster Segment polarity protein dishevelled (dsh), partial

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