Recombinant Drosophila melanogaster Selenide, water dikinase (SelD)

Code CSB-YP517720DLU
MSDS
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Source Yeast
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Code CSB-EP517720DLU
MSDS
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Source E.coli
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Code CSB-EP517720DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517720DLU
MSDS
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Source Baculovirus
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Code CSB-MP517720DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SelD
Uniprot No.
Alternative Names
SelD; PTF1; ptuf; CG8553; Inactive selenide; water dikinase-like protein; Protein patufet; dSelD
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-398
Target Protein Sequence
MSYAADVLNS AHLELHGGGD AELRRPFDPT AHDLDASFRL TRFADLKGRG CKVPQDVLSK LVSALQQDYS AQDQEPQFLN VAIPRIGIGL DCSVIPLRHG GLCLVQTTDF FYPIVDDPYM MGKIACANVL SDLYAMGVTD CDNMLMLLAV STKMTEKERD VVIPLIMRGF KDSALEAGTT VTGGQSVVNP WCTIGGVAST ICQPNEYIVP DNAVVGDVLV LTKPLGTQVA VNAHQWIDQP ERWNRIKLVV SEEDVRKAYH RAMNSMARLN RVAARLMHKY NAHGATDITG FGLLGHAQTL AAHQKKDVSF VIHNLPVIAK MAAVAKACGN MFQLLQGHSA ETSGGLLICL PREQAAAYCK DIEKQEGYQA WIIGIVEKGN KTARIIDKPR VIEVPAKD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in apoptosis and consequently a role in imaginal disk patterning and growth.
Gene References into Functions
  1. the selenophosphate synthetase 1 regulate PLP bisynthesis, and the intracellular PLP level affects various biological process such as amino acid metabolism, defense and other important metabolic activities. PMID: 21864351
  2. Our results strongly suggest that selD haploinsufficiency makes heterozygous flies more sensitive to oxidative stress and add further evidence to the role of selenoproteins as cellular antioxidants PMID: 12527370
  3. in Drosophila, SPS1/SelD regulates the intracellular glutamine by inhibiting GS1 and l(2)01810 expression PMID: 19755423
Protein Families
Selenophosphate synthase 1 family, Class II subfamily
Tissue Specificity
Expressed at low levels throughout the embryo. At later developmental stages, expressed in areas of high cell proliferation. From embryo stage 13, expression is high in central nervous system and midgut, especially in the gastric caeca. Expression also se
Database Links

KEGG: dme:Dmel_CG8553

STRING: 7227.FBpp0086691

UniGene: Dm.2979

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