Recombinant Drosophila melanogaster Serine protease snake (snk)

Code CSB-YP361452DLU
MSDS
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Source Yeast
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Code CSB-EP361452DLU
MSDS
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Source E.coli
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Code CSB-EP361452DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP361452DLU
MSDS
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Source Baculovirus
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Code CSB-MP361452DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
snk
Uniprot No.
Alternative Names
snk; CG7996; Serine protease snake; EC 3.4.21.-
Species
Drosophila melanogaster (Fruit fly)
Expression Region
28-435
Target Protein Sequence
LDA LEIINYQTTK YTIPEVWKEQ PVATIGEDVD DQDTEDEESY LKFGDDAEVR TSVSEGLHEG AFCRRSFDGR SGYCILAYQC LHVIREYRVH GTRIDICTHR NNVPVICCPL ADKHVLAQRI SATKCQEYNA AARRLHLTDT GRTFSGKQCV PSVPLIVGGT PTRHGLFPHM AALGWTQGSG SKDQDIKWGC GGALVSELYV LTAAHCATSG SKPPDMVRLG ARQLNETSAT QQDIKILIIV LHPKYRSSAY YHDIALLKLT RRVKFSEQVR PACLWQLPEL QIPTVVAAGW GRTEFLGAKS NALRQVDLDV VPQMTCKQIY RKERRLPRGI IEGQFCAGYL PGGRDTCQGD SGGPIHALLP EYNCVAFVVG ITSFGKFCAA PNAPGVYTRL YSYLDWIEKI AFKQH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo.
Gene References into Functions
  1. Mutagenesis of the cysteine-rich clip domain in the Drosophila patterning protease, Snake, is reported. PMID: 18477463
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family, CLIP subfamily
Database Links

KEGG: dme:Dmel_CG7996

STRING: 7227.FBpp0111746

UniGene: Dm.2435

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