Recombinant Drosophila melanogaster Sodium/potassium-transporting ATPase subunit alpha (Atpalpha), partial

Code CSB-YP321133DLU
MSDS
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Source Yeast
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Code CSB-EP321133DLU
MSDS
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Source E.coli
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Code CSB-EP321133DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321133DLU
MSDS
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Source Baculovirus
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Code CSB-MP321133DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Atpalpha
Uniprot No.
Alternative Names
Atpalpha; Na-p; CG5670Sodium/potassium-transporting ATPase subunit alpha; Na(+)/K(+) ATPase alpha subunit; EC 7.2.2.13; Sodium pump subunit alpha
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Gene References into Functions
  1. genome-wide screen to find regions of the genome containing elements important for genetic modulation of ATPalpha dysfunction, were identified. PMID: 25476251
  2. Data suggest that the sodium/potassium pump Na+/K+-ATPase alpha subunit may be a key target of the circadian pacemaker to impose daily control on brain activities. PMID: 24040028
  3. Gene expression of the alpha-subunit of Na+/K+-ATPase showed a robust daily rhythm in concentration changes. PMID: 19428365
  4. analysis of a series of new missense alleles of ATPalpha in Drosophila PMID: 19455355
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Tissue Specificity
High levels are found in some adult tissues: Malpighian tubules, indirect flight muscles, tubular leg muscles and throughout the nervous system (brain, optic lobes, retina and ventral thoracic neuromere). Lower levels are detected at the posterior end whe
Database Links

KEGG: dme:Dmel_CG5670

STRING: 7227.FBpp0088502

UniGene: Dm.6725

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