Recombinant Drosophila melanogaster Sperm-specific protein Don juan (dj)

Code CSB-YP519032DLU
MSDS
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Source Yeast
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Code CSB-EP519032DLU
MSDS
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Source E.coli
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Code CSB-EP519032DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519032DLU
MSDS
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Source Baculovirus
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Code CSB-MP519032DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dj
Uniprot No.
Alternative Names
dj; CG1980Sperm-specific protein Don juan
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-248
Target Protein Sequence
MFKRTALILR RCFQPTFIRP HHINVLENFK EADDLPNQGQ AKFVDVSIHD PQHIRSALVS PMQRKFLQDL EQQQTVRIKW FKEGNQDELE NMKNECRRLA LEIIMAAKGG DIKKACKELA EKEKCKQIEL KKKCKELEKK TKCAKKDPCK KKDPCKKKDP CKKKDPCKKK DPCKKKDPCK KKDPCKKKDP CKKKGGDLKK KCKKLAEKEK CKKLAKKEKM KKLQKKCKKM AQKEKCKKMA KKDKCKKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May be involved in the final steps of mitochondrial differentiation within the flagellum.
Gene References into Functions
  1. A new translational repression element and unusual transcriptional control regulate expression of don juan during Drosophila spermatogenesis. PMID: 11744372
  2. dj encodes proteins of the spermatid nucleus and the flagellum and is regulated at the transcriptional level by cannonball. PMID: 16477641
Subcellular Location
Nucleus. Mitochondrion. Note=Predominantly nuclear. Some fraction is associated with mitochondria.
Tissue Specificity
Expression limited to post-meiotic male germ cells. Expressed in elongated spermatids during individualization and in finally elongated nuclei of spermatids. After completion of nuclear shaping it is no longer expressed in the sperm heads with the onset o
Database Links

KEGG: dme:Dmel_CG1980

STRING: 7227.FBpp0088683

UniGene: Dm.36736

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