Recombinant Drosophila melanogaster Spindle assembly abnormal protein 6 homolog (sas-6)

Code CSB-YP892701DLU
MSDS
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Source Yeast
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Code CSB-EP892701DLU
MSDS
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Source E.coli
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Code CSB-EP892701DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892701DLU
MSDS
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Source Baculovirus
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Code CSB-MP892701DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sas-6
Uniprot No.
Alternative Names
Sas-6; SAS6; CG15524; Spindle assembly abnormal protein 6 homolog
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-472
Target Protein Sequence
MWPPGSEDSY SAKMDYGKSV VNILPSVEML VNFNGEMTRS SKRSCLLYAE RVDFKELLQL RLTEKSDQRR MYITTVDSAS FQDLKQDQSL NVSFSGFIDN VVRMLKDCQS GKLELHLTTR DQNLSSGREV HDYYLQFVEI RSFKNLVHLS LPCRSAPLNT VLFYINSMLE ASHKKQYILE QSMQQMQAEI NAQRAHAERL TTENTNIREA LAENTRILEE KHAAEVHQYQ EKLSKINEQR SNELERNRRA ISGFQAQLDK ASLEKAELKS AQEQAEKRCQ TLSEELSCCK ARVCTLKEQN DKLHGDVANI RKHERKLEYK IEDLKQHTVE LQEHIQKGNK EKANIAAELE AEKKILHTKR QALEMASEEI SKANQIIVKQ SQELLNLKKT IAWRTEVALQ QEKAVQAKES LLSLRENELR EARITIEKLR EEIPQQLQSM RNFAQGLEQK YSKQILILKE RLAIPTGKEN RR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication. In ciliated neurons, required for centriole assembly but not necessary for cilium basal body and cilia function. Required for sperm cilium basal body elongation together with Cep135 and Ana2.
Gene References into Functions
  1. These results indicate that centriole biogenesis does not strictly depend on SAS-6 self-assembly, and may require preexisting centrioles to ensure structural accuracy, fundamentally deviating from the current paradigm. PMID: 26609813
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body.
Tissue Specificity
Expressed in spermatocyte and speratids (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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