Recombinant Drosophila melanogaster Splicing factor U2AF 50 kDa subunit (U2af50)

Code CSB-YP636558DLU
MSDS
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Source Yeast
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Code CSB-EP636558DLU
MSDS
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Source E.coli
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Code CSB-EP636558DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP636558DLU
MSDS
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Source Baculovirus
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Code CSB-MP636558DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
U2af50
Uniprot No.
Alternative Names
U2af50; CG9998Splicing factor U2AF 50 kDa subunit; U2 auxiliary factor 50 kDa subunit; U2 snRNP auxiliary factor large subunit
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-416
Target Protein Sequence
MGYDDRERDR ERRRHRSRSR DRHRERSRDR RHHRNSRRKP SLYWDVPPPG FEHITPMQYK AMQASGQIPA SVVPDTPQTA VPVVGSTITR QARRLYVGNI PFGVTEEEMM EFFNQQMHLV GLAQAAGSPV LACQINLDKN FAFLEFRSID ETTQAMAFDG INLKGQSLKI RRPHDYQPMP GITDTPAIKP AVVSSGVIST VVPDSPHKIF IGGLPNYLND DQVKELLLSF GKLRAFNLVK DAATGLSKGY AFCEYVDLSI TDQSIAGLNG MQLGDKKLIV QRASVGAKNA QNAANTTQSV MLQVPGLSNV VTSGPPTEVL CLLNMVTPDE LRDEEEYEDI LEDIKEECTK YGVVRSVEIP RPIEGVEVPG CGKVFVEFNS VLDCQKAQQA LTGRKFSDRV VVTSYFDPDK YHRREF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Necessary for the splicing of pre-mRNA. Binds to the polypyrimidine tract of introns early during spliceosome assembly.
Gene References into Functions
  1. Reduction of U2AF50 expression promotes nuclear accumulation of expanded CAG RNA and enhances neurodegeneration. PMID: 21725067
  2. Sex-lethal splicing autoregulation in vivo: interactions between SEX-LETHAL, the U1 snRNP and U2AF underlie male exon skipping. PMID: 12490553
  3. The splicing factor U2AF50 is found to be involved with nuclear export of intronless mrnas. PMID: 15200955
Subcellular Location
Nucleus.
Protein Families
Splicing factor SR family
Database Links

KEGG: dme:Dmel_CG9998

STRING: 7227.FBpp0298005

UniGene: Dm.5430

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