Recombinant Drosophila melanogaster Synaptotagmin 1 (Syt1), partial

1. This study uncovered essential residues within Synaptotagmin that suggest a structural basis for several activities required for fusion, including a C2B domain surface implicated in SNARE complex interaction that is required for rapid synchronization and Ca(2+) cooperativity of vesicle release. PMID: 28895532
2. tethering of Syt1 to synaptic vesicles in vivo is a prerequisite for its role in facilitating fast synchronous synaptic vesicle release and suppressing asynchronous and spontaneous fusion PMID: 25775572
3. synaptic transmission can be regulated by Syt1 multimerization and that both C2 domains of Syt1 are uniquely required for modulating Ca(2+)-independent spontaneous fusion and Ca(2+)-dependent synchronous release. PMID: 23283333
4. effect of APP gene on synaptotagmin 1 mRNA level PMID: 22419087
5. The major function of Ca2+ binding to synaptotagmin's C2A domain is to neutralize the negative charge of the pocket, thereby unleashing the fusion-stimulating activity of synaptotagmin. PMID: 22279210
6. this study provided direct support for the hypothesis that plasma membrane penetration, specifically by the C(2)B domain of synaptotagmin, is the critical effector interaction for coupling Ca(2+) binding with vesicle fusion PMID: 21307261
7. Results suggest that the tandem C2 domains of Syt 1 play independent roles in neurotransmission. PMID: 20679236
8. The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo PMID: 12110842
9. Synaptotagmins I and IV promote transmitter release independently of Ca(2+) binding in the C(2)A domain PMID: 12110845
10. Data show that synaptotagmin I is required for a post-docking step during vesicle fusion but does not function to stabilize the docked vesicle state. PMID: 12467585
11. These results indicate that synaptotagmin is the major Ca(2+) sensor for evoked release and functions to trigger synchronous fusion in response to Ca(2+), while suppressing asynchronous release. PMID: 12467593
12. Syt I is necessary for the endocytosis of synaptic vesicles that have undergone exocytosis using a functional Syt I protein PMID: 14634669
13. synaptotagmin 1 is a negative regulator of vesicle fusion of synaptic transmission. PMID: 16061495
14. Elimination of synaptic transmission and decrease in Ca(2+) affinity of release observed in Ca(2+)-binding motif mutants is not due to altered synaptic vesicle distribution but rather is because of disrupting synaptotagmin I's ability to bind Ca(2+). PMID: 16528727
15. These results demonstrate that the polylysine motif is required for efficient Ca2+-independent docking and/or priming of synaptic vesicles in vivo. PMID: 16987956
16. Sr(2+) partially substitutes for Ca(2+) in synchronous release but does not support the negative regulatory function of Syt I. PMID: 17079341
17. Presence of two presynaptic calcium sensors in a biological context, one a calcium-based sensor devoted primarily to baseline synaptic transmission and a second sensor for short-term synaptic plasticity. PMID: 17709738
18. human APP gene plays role in synaptogenesis in transgenic lines of Drosophila melanogaster whose nerve cells express the human APP695 isoform, truncated APPs, and the presynaptic marker synaptotagmin driving the sequence of the green fluorescent protein. PMID: 19239106
19. Results demonstrate that synaptotagmin I plays a direct role in regulating spontaneous transmitter release, indicative of an active role in synaptic vesicle stabilization mediated by the C(2)A polylysine motif. PMID: 19358157

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KEGG: dme:Dmel_CG3139

STRING: 7227.FBpp0077410

UniGene: Dm.19478