Recombinant Drosophila melanogaster Syndecan (Sdc), partial

Code CSB-YP342222DLU
MSDS
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Source Yeast
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Code CSB-EP342222DLU
MSDS
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Source E.coli
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Code CSB-EP342222DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP342222DLU
MSDS
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Source Baculovirus
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Code CSB-MP342222DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sdc
Uniprot No.
Alternative Names
Sdc; Syd; CG10497; Syndecan
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell surface proteoglycan that bears heparan sulfate. Required for axonal and myotube guidance, is a necessary component of slit/robo signaling and is required in the slit target cells.
Gene References into Functions
  1. all detectable Sdc found at the NMJ is provided by the muscle, strongly suggesting a post-synaptic role for Sdc. both the cytoplasmic and extracellular domains of Sdc are required to promote synapse growth or to rescue Sdc loss of function. PMID: 26987116
  2. We conclude that Sdc cell autonomously regulates Slit/Robo2 signalling in tracheal cells to guarantee ordered directional migration and branch fusion. PMID: 21836636
  3. In this study, we provide evidence that Dlp and Sdc have both overlapping and distinct functions in axon guidance and in defining accurate patterns of axonal fasciculation within the lateral CNS neuropil PMID: 21500363
  4. Syndecan acting as a co-receptor for Slit in the Drosophila heart. PMID: 21565181
  5. Syndecan (Sdc) is critical for the fidelity of Slit repellent signaling at the midline of the Drosophila CNS. PMID: 15043815
  6. The heparan sulfate proteoglycan syndecan is an in vivo ligand for the Drosophila LAR receptor tyrosine phosphatase. PMID: 16213816

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Syndecan proteoglycan family
Tissue Specificity
In 13-16 hours embryos, expressed in lymph glands, peripheral and central nervous system and basal surfaces of gut epithelia. Sdc and robo are coexpressed in domains adjacent to slit; in tracheal pits and midline glia cells.
Database Links

KEGG: dme:Dmel_CG10497

STRING: 7227.FBpp0271715

UniGene: Dm.721

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