Recombinant Drosophila melanogaster T-box protein H15 (H15), partial

Code CSB-YP839101DLU
MSDS
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Source Yeast
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Code CSB-EP839101DLU
MSDS
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Source E.coli
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Code CSB-EP839101DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839101DLU
MSDS
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Source Baculovirus
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Code CSB-MP839101DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
H15
Uniprot No.
Alternative Names
H15; CG6604T-box protein H15
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. In odd-numbered abdominal segments, Mid and H15 activity plays an important role in restricting the expression of Wg to a single domain. PMID: 15458640
  2. In odd-numbered abdominal segments, Mid/H15 activity plays an important role in restricting the expression of Wg to a single domain. PMID: 15458640
  3. H15 participates in cardiac fate specification and may normally act redundantly with other cardiogenic factors. PMID: 15680363
  4. Data suggest that neuromancer 1 and 2 act both in concert with and subsequent to pannier and tinman in cardiac specification and differentiation. PMID: 15733676
  5. one of the major functions of mid and H15 during cardioblast development is the re-activation of tin expression at a stage when the induction of tin by Dpp in the dorsal mesoderm has ceased PMID: 15922573
  6. The function of the HI5 protein in Drosophila melanogaster neurogensis is described. PMID: 16499900
  7. Nmr1 and nmr2 appear to act together as members of the combinatorial code of transcription factors that govern neuronal subtype identity in the central nervous system. PMID: 19013145
  8. nmr is a gene required in adult fly hearts for the maintenance of both normal myofibrillar architecture and cardiac physiology. PMID: 19074289
  9. Results suggest that midline and H15 may be considered as the selector genes for ventral leg fate. PMID: 19605497

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG6604

STRING: 7227.FBpp0078710

UniGene: Dm.4780

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