Recombinant Drosophila melanogaster Trafficking kinesin-binding protein milt (milt), partial

Code CSB-YP846537DLU
MSDS
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Source Yeast
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Code CSB-EP846537DLU
MSDS
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Source E.coli
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Code CSB-EP846537DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846537DLU
MSDS
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Source Baculovirus
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Code CSB-MP846537DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
milt
Uniprot No.
Alternative Names
milt; CG43227; Trafficking kinesin-binding protein milt; Protein milton
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for kinesin-mediated axonal transport of mitochondria to nerve terminals. The oocyte acquires the majority of its mitochondria by competitive bidirectional transport along microtubules mediated by the Milton adapter. Mitochondria enter the young oocyte en mass from interconnected germ cells to generate the large aggregate known as the Balbiani body. Milt and Miro form an essential protein complex that links Khc to mitochondria for light chain-independent, anterograde transport of mitochondria.
Gene References into Functions
  1. Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain-independent, anterograde transport of mitochondria. PMID: 16717129
  2. Milton controls the early acquisition of mitochondria by Drosophila oocytes PMID: 16887820
Subcellular Location
Mitochondrion.
Tissue Specificity
Expressed primarily in axons and synapses.
Database Links

KEGG: dme:Dmel_CG43227

STRING: 7227.FBpp0297341

UniGene: Dm.7402

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