Recombinant Drosophila melanogaster Transcription initiation factor TFIID subunit 10b (Taf10b)

Code CSB-YP896858DLU
MSDS
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Source Yeast
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Code CSB-EP896858DLU
MSDS
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Source E.coli
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Code CSB-EP896858DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896858DLU
MSDS
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Source Baculovirus
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Code CSB-MP896858DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Taf10b
Uniprot No.
Alternative Names
Taf10b; Taf16; CG3069; Transcription initiation factor TFIID subunit 10b; Transcription initiation factor TFIID 16 kDa subunit; TAFII-16; TAFII16; dTAF(II)16
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-146
Target Protein Sequence
MVGSNFGIIY HNSAGGASSH GQSSGGGGGG DRDRTTPSSH LSDFMSQLED YTPLIPDAVT SHYLNMGGFQ SDDKRIVRLI SLAAQKYMSD IIDDALQHSK ARTHMQTTNT PGGSKAKDRK FTLTMEDLQP ALADYGINVR KVDYSQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.
Gene References into Functions
  1. Report shows the functional similarities and differences of TAF10 and TAF10b, orthologs of Drosophila melanogaster. In silico modeling suggest that dTAF10 and dTAF10b have similar secondary structures with a histone-fold domain, same interaction partners and similar expression patterns in neuronal tissues. Also, the loss of only dTaf10b causes lethality in the late pupal phase. PMID: 28841365
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TAF10 family
Tissue Specificity
At embryonic stage 9, expression is seen in the mesodermal layer and midgut primordia. The mesoderm-specific expression persists in later stages of development and at its highest level is detected in midgut, hindgut, and differentiating somatic muscle fib
Database Links

KEGG: dme:Dmel_CG3069

STRING: 7227.FBpp0077415

UniGene: Dm.21526

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